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Carbon Energy, EarlyView.The photocatalytic hydrogen evolution reaction (HER) activity of the polyoxometalate (POM)‐derived dual S‐scheme heterojunction photocatalyst WS2/Co9S8/ZnCdS reaches a value as high as 15.66 mmol g−1 h−1 under visible light (λ ≥ 420 nm), providing a straightforward approach for the development of dual S‐scheme heterojunctions to enhance the efficiency ...
Shuanghe Fu +12 more
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Light sheet fluorescence microscopy
Nature Reviews Methods Primers, 2021Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical sectioning capability and, hence, provide axial resolution, restrict photobleaching and phototoxicity to a fraction of the sample and use cameras to record tens to thousands of ...
Adam Glaser, Emmanuel G Reynaud
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Light-sheet fluorescence microscopy for quantitative biology
Nature Methods, 2014In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.
Ernst H K Stelzer
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Doubling the resolution of light-sheet fluorescence microscopy
Nature Methods, 2022openaire +4 more sources
Practical considerations for quantitative light sheet fluorescence microscopy
Nature Methods, 2022Fluorescence microscopy has evolved from a purely observational tool to a platform for quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic imaging modalities has spurred a rapid growth in light sheet fluorescence microscopy (LSFM).
Chad M. Hobson +5 more
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Fast Fluorescence Microscopy with Light Sheets
The Biological Bulletin, 2016In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of
Daetwyler, S., Huisken, J.
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Digital Scanned Laser Light Sheet Fluorescence Microscopy
Cold Spring Harbor Protocols, 2010INTRODUCTIONModern applications in the life sciences are frequently based on in vivo imaging of biological specimens, a domain for which light microscopy approaches are typically best suited. Often, quantitative information must be obtained from large multicellular organisms on the cellular or even subcellular level and with a good temporal resolution.
Philipp J, Keller, Ernst H K, Stelzer
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Light-sheet fluorescence microscopy with metalens
ODS 2020: Industrial Optical Devices and Systems, 2020We propose a Light-sheet fluorescent microscopy (LSFM) system with a flat metalens, which is used to replace the bulky illuminating components. The metalens is a diffractive optics elements (DOEs) composed of various gallium nitride (GaN) nanopillar for light-sheet generation, and it can be readily integrated in existing LSFM systems.
Cheng Hung Chu +6 more
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Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
Journal of Visualized Experiments, 2022Deafness is the most common sensory impairment, affecting approximately 5% or 430 million people worldwide as per the World Health Organization1. Aging or presbycusis is a primary cause of sensorineural hearing loss and is characterized by damage to hair cells, spiral ganglion neurons (SGNs), and the stria vascularis. These structures reside within the
Peter A, Santi, Shane B, Johnson
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Volume holographic optical element for light sheet fluorescence microscopy
Optics Letters, 2020Three-dimensional (3D) imaging of living organisms requires fine optical sectioning and high-speed image acquisition, which can be achieved by light sheet fluorescence microscopy (LSFM). However, orthogonal illumination and detection arms in the LSFM system make it bulky.
Ting-Yu, Hsieh +3 more
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