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Light-sheet fluorescent microscopy: fundamentals, developments and applications
Physica Scripta, 2023Abstract Deep volumetric microscopy of live objects plays a critical role in biology and medicine. To this end, development of rapid and non-invasive optical methods for 3-dimensional (3D) imaging is still demanding. In this way, light-sheet fluorescence microscopy (LSFM) has emerged as a volumetric microscopy method having high spatial ...
Hosein Kafian +3 more
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Metalens-based light-sheet fluorescence microscopy
Optica Imaging Congress 2025 (3D, DH, COSI, IS, pcAOP,RadIT)Metalens-based dual-sided illumination light-sheet fluorescence microscopy utilizes paired cylindrical metalenses to generate uniform twin light sheets, eliminating shadow artifacts. The mouse lung tissues are measured by the system to offer high-resolution and sectioning imaging results.
Hung-Chuan Hsu +9 more
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Light sheet fluorescence microscopy with active optical manipulation
Optics LettersWe present a light sheet fluorescence microscopy (LSFM) with active optical manipulation by using linear optical tweezers (LOTs). In this method, two coaxially transmitting laser beams of different wavelengths are shaped using cylindrical lenses to form a linear optical trapping perpendicular to the optical axis and an excitation light sheet (LS ...
Jun, Yin +4 more
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Light sheet fluorescence microscopy (LSFM): past, present and future
The Analyst, 2014Light sheet fluorescence microscopy (LSFM) has emerged as an important imaging modality to follow biology in live 3D samples over time with reduced phototoxicity and photobleaching.
John, Lim +3 more
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Multimodal light-sheet microscopy for fluorescence live imaging
SPIE Proceedings, 2012Light-sheet microscopy, it is known as single plane illumination microscope (SPIM), is a fluorescence imaging technique which can avoid phototoxic effects to living cells and gives high contrast and high spatial resolution by optical sectioning with light-sheet illumination in developmental biology.
Y. Oshima +2 more
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Smartphone light sheet fluorescence microscopy for molecular diagnostics
Biophotonics Congress: Optics in the Life Sciences Congress 2019 (BODA,BRAIN,NTM,OMA,OMP), 2019A new design and implementation of smartphone light sheet microscopy based on inkjet-printed DotLens is presented. Its ultimate simplicity, yet high quality imaging and sensing capabilities would find applications in point-of-care diagnostics and low-resources scenarios.
Hoang Nguyen, Wei-Chuan Shih
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Light-Sheet Fluorescence Microscopy
2022George O. T. Merces, Emmanuel G. Reynaud
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Metasurface-based Airy light-sheet fluorescence microscopy
Applied Physics ReviewsLight-sheet fluorescence microscopy (LSFM) is an essential imaging system for observing biological samples with high contrast, low phototoxicity, and rapid image acquisition. Traditional LSFM utilizes Gaussian illumination with a limited system field of view (FOV) due to the short Rayleigh range.
Hung-Chuan Hsu +8 more
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Light-sheet light-field fluorescence microscopy
Optics and Lasers in Engineering, 2022Dong Liang +6 more
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Light-Sheet Fluorescence Microscopy
Acta Optica Sinica, 2017杨豫龙 Yang Yulong +3 more
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