Results 111 to 120 of about 1,408,386 (261)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Influence of Light Quality on the Initial Development in Edible Brown Alga <i>Cladosiphon okamuranus</i>. [PDF]

Plants (Basel)
Sato Y, Inomata E, Nagoe H, Numata Y, Tanaka A.   +4 more
europepmc   +1 more source

Diversity of gene expression responses to light quality in barley. [PDF]

Sci Rep, 2023
Rodríguez Del Río Á, Monteagudo A, Contreras-Moreira B, Kiss T, Mayer M, Karsai I, Igartua E, Casas AM.   +7 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Effect of Different Light Quality and Photoperiod on Mycelium and Fruiting Body Growth of Tricholoma giganteum. [PDF]

Life (Basel)
Luo Q, Zhan M, Yan S, Xie T, Huang X, Wang R, Lu H, Wang S, Lin J.   +8 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Integrated transcriptomic and metabolomic analyses reveal light-quality-dependent regulation of specialized metabolism in Pseudostellaria heterophylla. [PDF]

BMC Genomics
Tang L, Yang Y, Jiang J, He H, Liu J, Xu Q, Shu G.   +6 more
europepmc   +1 more source

Light quality as a driver in adapting photosynthetic acclimation to niche partitioning. [PDF]

J Exp Bot, 2023
Kim E.
europepmc   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

LED Light-Quality Optimization to Enhance Shoot and Essential Oil Yield of <i>Tagetes erecta</i> L. in Controlled Environment. [PDF]

Molecules
Chu HTT, Nguyen NH, Phan Q, Dinh TTT, Hoang THT, Nguyen TV, Chu HH, Tong QC, Tien TQ, Setzer WN, Tran KQ, Do PT.   +11 more
europepmc   +1 more source