Imaging the developing human external and internal urogenital organs with light sheet fluorescence microscopy. [PDF]
, 2020 Technological advances in three-dimensional (3D) reconstruction techniques have previously enabled paradigm shifts in our understanding of human embryonic and fetal development. Light sheet fluorescence microscopy (LSFM) is a recently-developed technique
Baskin, Laurence +9 more
core
Sensorized Engineered Tissues with Built‐in Thermoregulation and Nutrient Supply
Advanced Functional Materials, EarlyView.This work introduces a granular hydrogel‐based tissue engineering platform that includes a closed‐loop temperature control to maintain 37°C and sustainably releases nutrients, thereby enabling cells to retain a high viability even if stored at room temperature for up to 24 h.
Antonia Georgopoulou +5 more
wiley +1 more source
Thin-sheet laser imaging microscopy for optical sectioning of thick tissues
BioTechniques, 2009 We report the development of a modular and optimized thin-sheet laser imaging microscope (TSLIM) for nondestructive optical sectioning of organisms and thick tissues such as the mouse cochlea, zebrafish brain/inner ear, and rat brain at a resolution that
Peter A. Santi +5 more
doaj +1 more source
Snapshot 3D tracking of insulin granules in live cells
, 2018 Rapid and accurate volumetric imaging remains a challenge, yet has the potential to enhance understanding of cell function. We developed and used a multifocal microscope (MFM) for 3D snapshot imaging to allow 3D tracking of insulin granules labeled with ...
Alan Selewa +8 more
core +1 more source
Global Connectivity and Function of Descending Spinal Input Revealed by 3D Microscopy and Retrograde Transduction [PDF]
, 2018 The brain communicates with the spinal cord through numerous axon tracts that arise from discrete nuclei, transmit distinct functions, and often collateralize to facilitate the coordination of descending commands.
Blackmore, Murray G. +4 more
core +2 more sources
Shaping of Biohybrid Functional Living Materials
Advanced Functional Materials, EarlyView.This work demonstrates a strategy for shaping living mycelium into functional materials by directing its natural growth. Nanoparticles armor hyphae, micron‐scale particles entangle within the network, and printed hydrogel architectures steer expansion, creating defined geometries.
Sarah Schyck +3 more
wiley +1 more source
Beam shaping in light-sheet microscopy: an experimental analysis
JPhys PhotonicsThanks to its unique optical sectioning capability, light-sheet fluorescence microscopy has proven to be a powerful technique for volumetric imaging of entire model organisms with high spatial and temporal resolution.
Manuel Hüpfel, Gerd Ulrich Nienhaus
doaj +1 more source
Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy
eLife, 2020 As the general population ages, more people are affected by eye diseases, such as retinopathies. It is therefore critical to improve imaging of eye disease mouse models.
Claudia Prahst +16 more
doaj +1 more source
Multiview deconvolution approximation multiphoton microscopy of tissues and zebrafish larvae
Scientific Reports, 2021 Imaging in three dimensions is necessary for thick tissues and small organisms. This is possible with tomographic optical microscopy techniques such as confocal, multiphoton and light sheet microscopy.
Dimitrios Kapsokalyvas +7 more
doaj +1 more source
Light-sheet fluorescence microscopy for biomedicine
, 2023 The research motivation behind this thesis is to expand the information that can currently be extracted from an ex vivo biological sample via light-sheet fluorescence microscopy (LSFM) by enhancing the achievable resolution at an increased penetration depth. LSFM has already been used considerably for various biomedical research applications.
openaire +3 more sources