Results 111 to 120 of about 136,404 (276)

Monitoring microbial communities using light sheet fluorescence microscopy [PDF]

Current Opinion in Microbiology, 2017
Microbes often live in dense, dynamic, multi-species communities whose architecture and function are intimately intertwined. Imaging these complex, three-dimensional ensembles presents considerable technical challenges, however. In this review, I describe light sheet fluorescence microscopy, a technique that enables rapid acquisition of three ...
openaire   +2 more sources

Multicolor 4D Fluorescence Microscopy using Ultrathin Bessel Light Sheets [PDF]

Scientific Reports, 2016
AbstractWe demonstrate a simple and efficient method for producing ultrathin Bessel (‘non-diffracting’) light sheets of any color using a line-shaped beam and an annulus filter. With this robust and cost-effective technology, we obtained two-color, 3D images of biological samples with lateral/axial resolution of 250 nm/400 nm and high-speed, 4D volume ...
Zhao, Teng, Lau, Sze Cheung, Wang, Ying, Su, Yumian, Wang, Hao, Cheng, Aifang, Herrup, Karl, Ip, Nancy Y., Du, Shengwang, Loy, M.M.T.   +9 more
openaire   +3 more sources

Artifact‐Minimizing Ultrathin Transparent Electrodes Fabricated via iCVD for In Vivo Optogenetic Stimulation and Neural Signal Monitoring of Primary Visual Cortex

Advanced Functional Materials, EarlyView.
We present ultrathin flexible transparent electrodes through iCVD‐enabled molecular control of 10 nm gold films on poly(dimethylaminomethylstyrene). In vivo validation demonstrated photoelectric artifact reduction vs. opaque electrodes and preservation of natural neural dynamics.
Tae Jin Mun, Minju Kim, Chaesung Kim, Yunyoung Choi, In‐Yeop Baek, Younghak Cho, Gyeongyong Lee, Yerim Jang, Min‐Ho Nam, Wonryung Lee, Maesoon Im, Hyejeong Seong   +11 more
wiley   +1 more source

Live cell imaging of low- and non-repetitive chromosome loci using CRISPR-Cas9. [PDF]

, 2017
Imaging chromatin dynamics is crucial to understand genome organization and its role in transcriptional regulation. Recently, the RNA-guidable feature of CRISPR-Cas9 has been utilized for imaging of chromatin within live cells. However, these methods are
Adli, Mazhar, Bandaria, Jigar, Darzacq, Xavier, Kuscu, Cem, Mir, Mustafa, Parlak, Mahmut, Qin, Peiwu, Singh, Ritambhara, Szlachta, Karol, Yildiz, Ahmet   +9 more
core   +1 more source

Mechanochromism of Glassy Polymers Enabled by a Loop‐Forming Supramolecular Mechanophore

Advanced Functional Materials, EarlyView.
The supramolecular mechanophore LOOP is covalently introduced into glassy polymers, capable of reporting force‐induced pre‐failure damage and local fracture events in polymer glasses. Under different mechanical deformation modes, including grinding, tensile stretching, and scratching, LOOP probes irreversible events in polymer glasses through a ...
Linlin Deng, Chantal Graafsma, Felix Meyenhofer, Iulia Scarlat, Luca Bertossi, Christoph Weder, Jess M. Clough   +6 more
wiley   +1 more source

Recent progress of second near-infrared (NIR-II) fluorescence microscopy in bioimaging

Frontiers in Physiology, 2023
Visualizing biological tissues in vivo at a cellular or subcellular resolution to explore molecular signaling and cell behaviors is a crucial direction for research into biological processes.
Tian Wang, Yingying Chen, Bo Wang, Mingfu Wu   +3 more
doaj   +1 more source

Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time [PDF]

, 2015
Fluorescence lifetime imaging (FLIM) combined with optical projection tomography (OPT) has the potential to map Förster resonant energy transfer (FRET) readouts in space and time in intact transparent or near transparent live organisms such as zebrafish ...
Alexandrov, Y, Andrews, N, Bugeon, L, Dallman, MJ, Frankel, P, French, PMW, Frolov, A, Kelly, DJ, Kerry, L, Kumar, S, Lockwood, N, McGinty, J, Ramel, M-C, Warren, SC   +13 more
core   +2 more sources

Strategies for Loading and Releasing Peptide Therapeutics in Biodegradable Carriers

Advanced Functional Materials, EarlyView.
A biodegradable carrier‐based peptide delivery system is a powerful treatment platform for diverse diseases, owing to its superior therapeutic efficacy and low toxicity. This review examines the conventional peptide‐loaded carrier fabrication process and its current limitations.
Wookyoung Jang, Ki Wan Bong
wiley   +1 more source

Amyloidogenic Peptide Fragments Designed From Bacterial Collagen‐like Proteins Form Hydrogel

Advanced Functional Materials, EarlyView.
This study identified amyloidogenic sequence motifs in bacterial collagen‐like proteins and exploited these to design peptides that self‐assemble into β‐sheet fibers and form hydrogels. One hydrogel supported healthy fibroblast growth, showing promise for biocompatible materials. Our work demonstrates that bacterial sequences can be harnessed to create
Vamika Sagar, Xuen J Ng, Lukas Lechel, Ashish Chand Rajwar, Hendrik Bargel, Abhishek A. Jalan   +5 more
wiley   +1 more source

Keeping Cells Alive in Microscopy

Biophysica
Light microscopy has emerged as one of the fundamental methods to analyze biological systems; novel techniques of 3D microscopy and super-resolution microscopy (SRM) with an optical resolution down to the sub-nanometer range have recently been realized ...
Herbert Schneckenburger, Christoph Cremer   +1 more
doaj   +1 more source