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Light sheet fluorescence microscopy
Nature Reviews Methods Primers, 2021Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical sectioning capability and, hence, provide axial resolution, restrict photobleaching and phototoxicity to a fraction of the sample and use cameras to record tens to thousands of ...
Adam Glaser, Emmanuel G Reynaud
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Practical considerations for quantitative light sheet fluorescence microscopy
Nature Methods, 2022Fluorescence microscopy has evolved from a purely observational tool to a platform for quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic imaging modalities has spurred a rapid growth in light sheet fluorescence microscopy (LSFM).
Chad M. Hobson +5 more
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Fast Fluorescence Microscopy with Light Sheets
The Biological Bulletin, 2016In light sheet microscopy, optical sectioning by selective fluorescence excitation with a sheet of light is combined with fast full-frame acquisition. This illumination scheme provides minimal photobleaching and phototoxicity. Complemented with remote focusing and multi-view acquisition, light sheet microscopy is the method of choice for acquisition of
Daetwyler, S., Huisken, J.
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Digital Scanned Laser Light Sheet Fluorescence Microscopy
Cold Spring Harbor Protocols, 2010INTRODUCTIONModern applications in the life sciences are frequently based on in vivo imaging of biological specimens, a domain for which light microscopy approaches are typically best suited. Often, quantitative information must be obtained from large multicellular organisms on the cellular or even subcellular level and with a good temporal resolution.
Philipp J, Keller, Ernst H K, Stelzer
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Light-sheet fluorescence microscopy with metalens
ODS 2020: Industrial Optical Devices and Systems, 2020We propose a Light-sheet fluorescent microscopy (LSFM) system with a flat metalens, which is used to replace the bulky illuminating components. The metalens is a diffractive optics elements (DOEs) composed of various gallium nitride (GaN) nanopillar for light-sheet generation, and it can be readily integrated in existing LSFM systems.
Cheng Hung Chu +6 more
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Imaging the Aging Cochlea with Light-Sheet Fluorescence Microscopy
Journal of Visualized Experiments, 2022Deafness is the most common sensory impairment, affecting approximately 5% or 430 million people worldwide as per the World Health Organization1. Aging or presbycusis is a primary cause of sensorineural hearing loss and is characterized by damage to hair cells, spiral ganglion neurons (SGNs), and the stria vascularis. These structures reside within the
Peter A, Santi, Shane B, Johnson
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Volume holographic optical element for light sheet fluorescence microscopy
Optics Letters, 2020Three-dimensional (3D) imaging of living organisms requires fine optical sectioning and high-speed image acquisition, which can be achieved by light sheet fluorescence microscopy (LSFM). However, orthogonal illumination and detection arms in the LSFM system make it bulky.
Ting-Yu, Hsieh +3 more
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Light-sheet fluorescence microscopy for quantitative biology
Nature Methods, 2014In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation process minimizes fluorophore bleaching and phototoxic effects. Because biological specimens survive long-term three-dimensional imaging at high spatiotemporal resolution, LSFM has become the tool of choice in developmental biology.
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Light sheet fluorescence microscopy with active optical manipulation
Optics LettersWe present a light sheet fluorescence microscopy (LSFM) with active optical manipulation by using linear optical tweezers (LOTs). In this method, two coaxially transmitting laser beams of different wavelengths are shaped using cylindrical lenses to form a linear optical trapping perpendicular to the optical axis and an excitation light sheet (LS ...
Jun, Yin +4 more
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Light sheet fluorescence microscopy (LSFM): past, present and future
The Analyst, 2014Light sheet fluorescence microscopy (LSFM) has emerged as an important imaging modality to follow biology in live 3D samples over time with reduced phototoxicity and photobleaching.
John, Lim +3 more
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