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Lignocellulolytic enzyme production from submerged fermentation of paddy straw [PDF]
Five strains of cellulolytic bacteria and four strains of Phanerochaete chrysosporium were evaluated for the lignocellulolytic enzyme production during submerged fermentation (SmF) of paddy straw. Extra-cellular enzyme assay for CMCase, FPase, Cellobiase, Xylanase, Lignin peroxidase and Laccase enzymes was performed after 7 and 15 days of submerged ...
B K, Mishra, A K, Pandey Lata
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Sustainable Production of Fungal Lignocellulolytic Enzyme Cocktails
2022Fungi can degrade lignocellulolytic materials by secreting complex enzyme cocktails composed of cellulolytic and lignolytic enzymes. In this work, seven fungal species (Mucor indicus DSM 2185, Paecilomyces variotii CBS 372.70, Myceliophthora thermophila CBS 663.74, Thielavia terrestris CBS 456.75, Botryosphaeria dothidea JCM 2738, Fusarium oxysporum f ...
Marđetko, Nenad +8 more
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Enhancing digestibility of Miscanthus using lignocellulolytic enzyme produced by Bacillus
Bioresource Technology, 2017In this study an effective bacterial pretreatment method was developed to improve digestibility of Miscanthus. Seven new bacterial isolates, which showed excellent xylanase production ability using Miscanthus as carbon source, were used to perform the pretreatment experiments.
Haipeng, Guo +7 more
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Involvement of Lignocellulolytic Enzymes in the Decomposition of Leaf Litter in a Subtropical Forest
Journal of Eukaryotic Microbiology, 2006ABSTRACT. The involvement of ligninolytic and cellulolytic enzymes, such as laccase, lignin peroxidase, manganese peroxidase, carboxymethylcellulase (CMCase), and filter paper activity (FPA), in the decomposition process of leaf litter driven by 6 soil‐inhabiting fungi imperfecti was studied under solid‐state fermentations.
Jie-Jie, Hao +5 more
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Protoplast fusion enhances lignocellulolytic enzyme activities in Trichoderma reesei
Biotechnology Letters, 2014Protoplast fusion was used to obtain a higher production of lignocellulolytic enzymes with protoplast fusion in Trichoderma reesei. The fusant strain T. reesei JL6 was obtained from protoplast fusion from T. reesei strains QM9414, MCG77, and Rut C-30. Filter paper activity of T. reesei JL6 increased by 18% compared with that of Rut C-30. β-Glucosidase,
Yu-xiao, Cui +6 more
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Heterologous Expression of Lignocellulolytic Enzymes in Aspergillus niger
2018Aspergillus niger is a genetically tractable model organism for scientific discovery and a platform organism used for the production of heterologous enzymes. Academic strategies to increase production include the use of strong promoters, multiple gene copies, gene knock out and knock in strains, secretion pathway engineering, and multi omics-based ...
Jinxiang Zhang +2 more
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Screening of Recombinant Lignocellulolytic Enzymes Through Rapid Plate Assays
2020In the search for novel biomass-degrading enzymes through mining microbial genomes, it is necessary to apply functional tests during high-throughput screenings, which are capable of detecting enzymatic activities directly by way of plate assay. Using the most efficient expression systems of Escherichia coli and Pichia pastoris, the production of a high
Anthi, Karnaouri +3 more
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Comparison of Mono- and Dikaryotic Medicinal Mushrooms Lignocellulolytic Enzyme Activity
International Journal of Medicinal Mushrooms, 2019Mono- and dikaryotic medicinal mushroom strains isolated from four wood-rotting basidiomycete fruiting bodies were comparatively evaluated for laccase, manganese peroxidase, cellulase, and xylanase activities in submerged cultivation in glucose or mandarin peel-containing media.
Eva, Kachlishvili +4 more
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Screening For Lignocellulolytic Enzymes-producing White Rot Fungi
2023Introduction: The three major parts of lignocellulose are cellulose, hemicellulose and lignin which can be broken down by cellulase, xylanase and laccase, respectively, thereby making the reducing sugar in lignocellulose available for industrial processes.Aims: This work aimed to screen for white-rot fungi with the potential of producing cellulase ...
Adedayo Fasiku, Samuel +2 more
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