Results 91 to 100 of about 5,522,817 (294)
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
, 2014 Systems of self-gravitating fermions constitute a topic of great interest in astrophysics, due to the wide field of applications. In this paper, we consider the gravitational equilibrium of spherically symmetric Newtonian models of collisionless ...
Alberti, Giuseppe, Merafina, Marco
core +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Bargaining with Many Players: A Limit Result [PDF]
We provide a simple characterization of the stationary subgame perfect equilibrium of an alternating offers bargaining game when the number of players increases without a limit.
Hannu Vartiainen, Klaus Kultti
core
Geomorphology, 2018 Understanding the impact of data uncertainty is a fundamental part of ensuring safe design of manmade excavations. Although good levels of knowledge are achievable from field investigations and experience, a natural geological environment is subject to ...
Claudio Vanneschi +5 more
semanticscholar +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
, 2002 A numerical technique is introduced that reduces exponentially the time required for Monte Carlo simulations of non-equilibrium systems. Results for the quasi-stationary probability distribution in two model systems are compared with the asymptotically ...
A. Bandrivskyy +9 more
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Optimization re-digging waste dump on exploitation Air Laya coal mine in South Sumatera, Indonesia
Journal of Degraded and Mining Lands ManagementIn doing slope design, paying attention to slope geometry and soil shear strength is necessary. Determination of slope geometry design also considers the balance between height and slope and production benefits.
Rosihan Pebrianto +3 more
doaj +1 more source
Analysis of conventional and helical soil nails using finite element method and limit equilibrium method. [PDF]
Heliyon, 2022 Goyal A, Shrivastava AK.
europepmc +1 more source