Results 151 to 160 of about 2,038,928 (302)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Lipid profiling of yeast RAD deletions, identification of lipid gene responsible for lipid accumulation and its transcriptional regulation.

, 2016
The study uncovers a phenomenon where lipid accumulation could be one of the markers for prematurely aging yeast. Along with that, a new role for a yeast TF and its target lipid gene in non-polar lipid biosynthesis are also ...
Kanaga Vijayan, D.
core   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Lipid-laden partially-activated plasmacytoid and CD4-CD8a+ dendritic cells accumulate in tissues in elderly mice

, 2014
Background - Aging is associated with a decline in lymphocyte function however, little is known about dendritic cell (DC) subsets and aging. Aging is also associated with increasing circulating lipid levels and intracellular lipid accumulation modulates ...
Danielle Dye (20078625), Cyril Mamotte (565952), Connie Jackaman (337770), T. McGonigle (23423203), Joanne Gardner (23423200), Delia Nelson (23307649)   +5 more
core  

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Maximizing lipid accumulation in Tetradesmus obliquus under heavy metal stress for sustainable biodiesel innovation

BMC Biotechnology
This study investigates the potential of Tetradesmus obliquus for lipid accumulation under heavy metal stress and evaluates it’s aviability for biodiesel production.
Eman A. Alwaleed, Hamdy R. M. Galal, Mohamed Aboueldahab, Hani Saber   +3 more
doaj   +1 more source

Cell surface CD11c as a neutrophil aging marker molecule

FEBS Open Bio, EarlyView.
Cell surface CD11chi neutrophils were more aged and had better phagocytic function than CD11c−/lo neutrophils. Transcriptomic analysis of CD11chi neutrophils and CD11c−/lo neutrophils in pediatric population showed that the most difference was seen in infants.
Sophia Koutsogiannaki, Fahd Alhamdan, Erik Malm, Marie Bermudez, Samuel Y. Kim, Koichi Yuki   +5 more
wiley   +1 more source

Dehydration promotes intracellular lipid synthesis and accumulation

Nature Communications
Lipids can be considered a water reservoir used to offset dehydration stress as their oxidation by the mitochondria generates water. However, whether dehydration and the ensuing hypertonic stress directly regulate lipid synthesis is unknown. We show that
Joshua S. Carty, Jazlyn Selvasingh, Yvonne Zuchowski, Hyuck-Jin Nam, Clothilde Pénalva, Gayani Nanayakkara, Erin Q. Jennings, Kelsey Voss, Edith T. Adame, John T. Tossberg, Wei Sheng Yap, Meiling Melzer, Olga Viquez, A. Scott McCall, Elizabeth R. Piotrowski, Ryoichi Bessho, Shirong Cao, Katrina L. Leaptrot, Alexandra C. Schrimpe-Rutledge, Simona G. Codreanu, Stacy D. Sherrod, John A. McLean, Jonathan B. Trapani, Matthew A. Cottam, Ma Wan, Deepti Shrivastava, Don A. Delker, Matthew H. Wilson, Clinton M. Hasenour, Louise Lantier, Irene Chernova, Jamey D. Young, Volker H. Haase, Jose Pablo Vazquez-Medina, Dylan K. Kosma, Peter K. Kim, Jean-Philippe Cartailler, Mingzhi Zhang, Roy Zent, Raymond C. Harris, Jason A. Watts, Andrew S. Terker, Fabian Bock, Jeffrey C. Rathmell, Aylin R. Rodan, Juan P. Arroyo   +45 more
doaj   +1 more source