Results 71 to 80 of about 843,571 (310)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

High-performance liquid chromatography of Group 6 dimetal decacarbonyl complexes singly-bridged by diphosphine ligands

, 1994
The normal-phase high-performance liquid chromatography of a series of Group 6 dimetal decacarbonyl complexes, of the form (OC)5M(P(CH2)(n)P)M(CO)5, where M = Cr, Mo and W, and P(CH2)(n)P = diphenylphosphinyl bridge with n = 2-6, is reported ...
Hor, T. S A, Lee, H. K., Hor, T.S.A., Tan, K.-C., Tan, K. C., Lee, H.K.   +5 more
core   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

FEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu, Lanlan Li, Wenjuan Jia, Hanzhong Feng, Xiaoquan Yu, Youli Zhao, Fengzhi Yuan, Hui Ding, Junqiang Tian, Yong‐Xing He, Zhiping Wang   +10 more
wiley   +1 more source

Separation of whey proteins for chromatography liquid

Scientia Agropecuaria, 2010
This paper describes and compares three chromatographic methods for the analysis and quantification of most abundant proteins in cheese whey, -lactalbumin and -lactoglobulin. The methods were: Reverse-phase high performance liquid chromatography, anion
Abraham D. Giraldo Zuñiga, Edwin E.García Rojas, Jane S. R.Coimbra, Wilmer E. Luera Peña   +3 more
doaj   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection

, 2013
Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma.
Pintus, Gianfranco, Angelo Zinellu, Erre, Gian Luca, Carru, Ciriaco, Salvatore Sotgia, Ciriaco Carru, Sotgia, Salvatore, Erre GL, Gianfranco Pintus, Zinellu, Angelo, Deiana, Luca, Gerard Aime Pinna, Luca Deiana, Pisanu, Elisabetta, Elisabetta Pisanu, Gian Luca Erre, Pinna, Gérard Aimé, Sotgia S   +17 more
core   +1 more source

TRIGLYCERIDES | Liquid Chromatography

, 2000
Peer ...
Ruiz-Gutiérrez, Valentina, Perona, Javier S.   +1 more
openaire   +2 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Advanced modelling and visualisation of liquid-liquid separations of complex sample components, with variable phase distribution and mode of operation

, 2013
This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.This research is about liquid-liquid chromatography modelling.
De Folter, Jozefus Johannes Martinus
core  

Analysis of Cordyceps by multi-column liquid chromatography

Acta Pharmaceutica Sinica B, 2017
Cordyceps is a famous traditional Chinese medicine (TCM) that has been used in China for hundreds of years. In the present study a multi-column liquid chromatography (MC-LC) system was developed for the qualitative analysis of macromolecules and ...
Zhengming Qian, Shaoping Li
doaj   +1 more source