Results 51 to 60 of about 712,786 (306)
FEBS Letters, EarlyView.Ascidian Ciona larvae initially show strong clockwise tail twisting, which is largely corrected during development. However, a small residual twist remains. This study shows that organized helical myofibrils in tail muscles mechanically stabilize this residual asymmetry, preventing complete restoration of bilateral symmetry and revealing how embryos ...
Yuki S. Kogure +3 more
wiley +1 more source
Journal of Family Medicine and Primary Care, 2020 Background: Early diagnosis and treatment of drug-resistant tuberculosis (TB) is crucial to halt the spread of drug resistance in the community. Aim: The aim of the study was to compare rapid diagnostic tests (GeneXpert and line probe assay, LPA) with ...
Vimal Kumar +8 more
doaj +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
Sporulation of Aspergillus niger in Submerged Liquid Culture [PDF]
Journal of General Microbiology, 1969 SUMMARY: Asexual sporulation of Aspergillus niger occurred in submerged culture in a liquid minimal medium without added nitrogen, in low ammonium-N concentrations, and in a wide range of nitrate-N concentrations. Ammonium salts containing more than 48 mg. atom N/l. were inhibitory to conidiation.
J C, Galbraith, J E, Smith
openaire +2 more sources
FEBS Letters, EarlyView.An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley +1 more source
Agronomy, 2020 Lingonberry (Vaccinium vitis-idaea L.) is a health-promoting small fruit crop rich in antioxidant metabolites that helps to reduce the incidence of degenerative diseases.
Samir C. Debnath, Usha Arigundam
doaj +1 more source
Culture of Human Keratinocytes in Liquid Medium
Journal of Investigative Dermatology, 1983 Since 1967, major advances have been made in procedures to isolate and maintain keratinocytes in liquid medium. Human keratinocytes and those from several laboratory animal species may now be isolated from skin either by direct trypsinization of minces, from split-thickness skin following the separation of the epidermis from the dermis, or from the ...
openaire +2 more sources
Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes
FEBS Letters, EarlyView.Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez +2 more
wiley +1 more source
FEBS Letters, EarlyView.Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley +1 more source
Novel 3D-Printed Cell Culture Inserts for Air–Liquid Interface Cell Culture
Life, 2022 In skin research, widely used in vitro 2D monolayer models do not sufficiently mimic physiological properties. To replace, reduce, and refine animal experimentation in the spirit of ‘3Rs’, new approaches such as 3D skin equivalents (SE) are needed to ...
Magdalena Bauer +5 more
doaj +1 more source