Results 41 to 50 of about 16,007 (302)

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

open access: yesMolecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen   +11 more
wiley   +1 more source

Evaluation of electroosmotic pumping effect in microporous media flow [PDF]

open access: yes, 2011
This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece.
Li, B   +2 more
core  

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

open access: yesFEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso   +4 more
wiley   +1 more source

The Universal Cloud and Aerosol Sounding System (UCASS): a low-cost miniature optical particle counter for use in dropsonde or balloon-borne sounding systems [PDF]

open access: yes, 2019
© Author(s) 2019. This work is distributed under the Creative Commons Attribution 4.0 License. An earlier version of this work was published in Atmospheric Measurement Techniques Discussions: https://dx.doi.org/10.5194/amt-2019-70.A low-cost miniaturized
Girdwood, Joseph   +11 more
core   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Study on Vapor Pressure of Liquid Desiccants Solution

open access: yesZhileng xuebao, 2004
The vapor pressure of desiccants solution has great impact on the performance of the liquid desiccant air-conditioning system. Calcium chloride is the cheapest and most readily available desiccant, but it has the disadvantage of higher vapor pressure ...
孙健, 宫小龙, 施明恒
doaj   +2 more sources

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

Hygrothermal simulation-informed design of mesoporous desiccants for optimised energy efficiency of mixed mode air conditioning systems [PDF]

open access: yes, 2015
This paper describes an optimization technique using hygrothermal numerical modelling to determine an ideal and unknown isotherm in order to inform the design of optimised mesoporous desiccants. Their suitability for passive humidity buffering as well as
Akiyama   +62 more
core   +2 more sources

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

open access: yesFEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart   +7 more
wiley   +1 more source

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