Results 151 to 160 of about 1,563,028 (315)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

A Multicriteria Analysis to Integrate Stakeholder Perceptions of Ecosystem-Based Flood Adaptations in Coastal Urban Areas

Documents d'Anàlisi Geogràfica
Inland and coastal floods are becoming more frequent and severe, affecting natural and socioeconomic systems. Local adaptation to climate change involves complex decisions which benefit from the integration of opinions from different stakeholders in the
Mar Riera-Spiegelhalder, Luís Campos Rodrigues, Adrián Ferrandis Martínez   +2 more
doaj   +1 more source

Structural variation and introgression from wild populations in East Asian cattle genomes confer adaptation to local environment. [PDF]

Genome Biol, 2023
Xia X, Zhang F, Li S, Luo X, Peng L, Dong Z, Pausch H, Leonard AS, Crysnanto D, Wang S, Tong B, Lenstra JA, Han J, Li F, Xu T, Gu L, Jin L, Dang R, Huang Y, Lan X, Ren G, Wang Y, Gao Y, Ma Z, Cheng H, Ma Y, Chen H, Pang W, Lei C, Chen N.   +29 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Rhizosphere microbial community assembly and association networks strongly differ based on vegetation type at a local environment scale. [PDF]

Front Microbiol, 2023
Liu L, Ma L, Zhu M, Liu B, Liu X, Shi Y.
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The scalar politics of economic planning [PDF]

, 2011
Across England, modes of governing larger-than-local development strategies are undergoing far-reaching change. In particular, the new government of 2010 has a political and financial mission of rescaling and simplifying sub-national economic planning ...
Townsend, Alan, Pugalis, L, Townsend, AR, Pugalis, Lee   +3 more
core  

Ca Cations Impact the Local Environment inside HZSM-5 Pores during the Methanol-to-Hydrocarbons Reaction. [PDF]

ACS Catal, 2023
Liutkova A, Zhang H, Simons JFM, Mezari B, Mirolo M, Garcia GA, Hensen EJM, Kosinov N.   +7 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Proximity biotinylation to define the local environment of the protein kinase A catalytic subunit in adrenal cells. [PDF]

STAR Protoc, 2023
Omar MH, Lauer SM, Lau HT, Golkowski M, Ong SE, Scott JD.   +5 more
europepmc   +1 more source