Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
Compliance and coloniality: aid bureaucracy and the failures of 'localisation' in Myanmar's complex emergency. [PDF]
Décobert A, Maung PP, Scott A, Wells T.
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Identification and Validation of Mannose Metabolism-Related Biomarkers in COPD Through Integrated Bioinformatics and Machine Learning Analysis: A Pilot Study. [PDF]
Li X, Wang J, Hu Z, Chen S, Liu C.
europepmc +1 more source
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley +1 more source
EXPRESS: Preclinical in-vivo cerebrovascular imaging in rodent models: Methods and applications in Alzheimer's Disease. [PDF]
Patalwala D +9 more
europepmc +1 more source
Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima +7 more
wiley +1 more source
Surgically Treated Non-Unions and Fractures of the Femur and Tibia in Adults: A Nationwide Analysis of 888,442 Cases from the German InEK Database. [PDF]
Graefe N +7 more
europepmc +1 more source
Large language models in respiratory care: safety and clinical integration. [PDF]
Porcella A +3 more
europepmc +1 more source
Improvements in high-yield detection and localisation of cholesterol in murine brains using water cluster beam secondary ion mass spectrometry. [PDF]
Lagator M +3 more
europepmc +1 more source

