Results 111 to 120 of about 22,242 (303)
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein
FEBS Open Bio, EarlyView.Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley +1 more source
The Syntax of Speech Acts: Deictic Inversion as an Evidential Strategy in English
LanguagesThis paper presents empirical evidence to support the so-called syntactization of discourse, that is, the projection of relevant pragmatic features in the narrow syntax.
Ana Ojea
doaj +1 more source
Aurelia Mallya: Locative-subject alternation constructions in Kiwoso
Ghana Journal of Linguistics, 2020 Locative subject alternation constructions show variation within and across languages in terms of subject agreement pattern and the type of predicates involved. In Kiwoso, the preverbal locative DPs with and without locative morphology are best analysed as canonical subjects, as evidenced by the subject diagnostics, such as subject-verb agreement and ...
openaire +2 more sources
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Embodied memory and curatorship in children’s digital video production [PDF]
, 2010 Digital video production in schools is often theorised, researched and written about in two ways: either as a part of media studies practice or as a technological innovation, bringing new, “creative”, digital tools into the curriculum.
Potter, John
core +2 more sources
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
LinguisticsAnalyzing the diachrony of Hebrew bein, originally a spatial preposition ‘within/among’, this article traces several grammaticization clines associated with bein’s gradual evolution into a rich variety of functions within various constructions. While its
Bardenstein Ruti, Ariel Mira
doaj +1 more source
Corpus of Mandarin Child Language: a preliminary study on the acquisition of semantic content categories in Mandarin-speaking preschoolers. [PDF]
Front Psychol, 2023 Tang TP, Lau DK, Leung MT.
europepmc +1 more source