Results 121 to 130 of about 1,436 (295)
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
Locative inversion in Otjiherero : more on morphosyntactic variation in Bantu
, 2013 This paper discusses locative inversion constructions in Otjiherero against the background of previous work by Bresnan and Kanerva (1989) on the construction in Chichewa, and Demuth and Mmusi (1997) on Setswana and related languages.
Marten, Lutz
core
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Textual Features of Locative Circumstances
, 2006 处所环境是小句背景信息的语义表征,大多由处所介词短语加以体现。从语篇角度来看,处所环境有自身的分布规律,从而建构特定的语篇特征。在药品服用方法和旅游城市概况两类语篇中,处所环境的选择存在一定的语类差异,相关的分布计量统计支持了上述的观点。Locative circumstance is the semantic representation of background information in a clause.
魏本力
core
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
, 2016 This study is an investigation on the spatial span of Persian prepositions in colloquial speech, focusing on the locative use of prepositions. The results are compared with English prepositions as well.
Moltaji, Niloofar
core
FEBS Open Bio, EarlyView.Despite frogs avoiding low temperatures, examination of four salamander species revealed that none avoided cold and all possessed cold tolerance. Functional analysis of TRPM8, a cold sensor, showed that all salamander TRPM8s had lost their cold sensitivity.
Tadahiro Sawao +3 more
wiley +1 more source
Locative expressions in signed languages: A cross-linguistic comparison
, 2013 The primary focus of this paper is to examine whether sign languages organize their locative expressions similarly to spoken languages. Paving the way in the study of spatial relations by focusing on the structuring of ON and IN locatives in spoken ...
Eberle, Sarah E.
core