Results 91 to 100 of about 296,746 (277)

Efficient methods for read mapping

, 2022
DNA sequencing is the mainstay of biological and medical research. Modern sequencing machines can read millions of DNA fragments, sampling the underlying genomes at high-throughput. Mapping the resulting reads to a reference genome is typically the first
Zhang, Haowen
core  

Reading music modifies spatial mapping in pianists [PDF]

, 2004
We used a novel musical Stroop task to demonstrate that musical notation is automatically processed in trained pianists. Numbers were superimposed onto musical notes, and participants played five-note sequences by mapping from numbers to fingers instead ...
Stewart, Lauren, Lauren Stewart, Walsh, V., Frith, U., Vincent Walsh, Stewart, L.; id_orcid, Walsh, Vincent, Uta Frith, Frith, Uta   +8 more
core   +1 more source

Mapping human pre-rRNA processing and modification at single nucleotide resolution using long read nanopore sequencing

Nature Communications
Ribosome biogenesis requires the synthesis and sequential processing of precursor rRNAs (pre-rRNAs) into mature rRNAs. Traditional methods such as northern blotting and metabolic labeling provide limited resolution.
Stefan Pastore, Ludivine Wacheul, Lioba Lehmann, Stefan Mündnich, Beat Lutz, Mark Helm, Susanne Gerber, Denis L. J. Lafontaine, Tamer Butto   +8 more
doaj   +1 more source

A comparison of short-read, HiFi long-read, and hybrid strategies for genome-resolved metagenomics

Microbiology Spectrum
Shotgun metagenomics enables the reconstruction of complex microbial communities at a high level of detail. Such an approach can be conducted using both short-read and long-read sequencing data, as well as a combination of both.
Raphael Eisenhofer, Joseph Nesme, Luisa Santos-Bay, Adam Koziol, Søren Johannes Sørensen, Antton Alberdi, Ostaizka Aizpurua   +6 more
doaj   +1 more source

Hippo pathway at the crossroads of stemness and therapeutic resistance in breast cancer

Molecular Oncology, EarlyView.
Dysregulation of the Hippo pathway drives nuclear accumulation of YAP/TAZ, activating stemness‐related transcriptional programs that sustain breast cancer stemness and fuel therapeutic resistance across subtypes, underscoring Hippo signaling as a targetable vulnerability. Figure created and edited with BioRender.com.
Giulia Schiavoni, Antonella Palmese, Stefano Scalera, Laura Cipriani, Davide Mascolo, Patrizia Vici, Teresa Arcuri, Lorena Filomeno, Eriseld Krasniqi, Giovanni Blandino, Giulia Bon, Marcello Maugeri‐Saccà   +11 more
wiley   +1 more source

Towards population-scale long-read sequencing

, 2021
: Long-read sequencing technologies have now reached a level of accuracy and yield that allows their application to variant detection at a scale of tens to thousands of samples.
Sedlazeck, Fritz J., De Coster, Wouter, Weissensteiner, Matthias H.   +2 more
core  

Decoding the human PBMC isonome: isoform-level resolution with single-cell long-read transcriptomics

Frontiers in Genetics
Long-read single-cell RNA sequencing provides an opportunity to understand human health and disease at a level difficult to resolve with bulk or short-read methods.
Patricia Hayes Doyle, Patricia Hayes Doyle, Madeline L. Page, Madeline L. Page, Madeline L. Page, J. Anthony Brandon, J. Anthony Brandon, J. Anthony Brandon, Bernardo Aguzzoli Heberle, Bernardo Aguzzoli Heberle, Brendan J. White, Ann Marie Stowe, Ann Marie Stowe, Ann Marie Stowe, Mark T. W. Ebbert, Mark T. W. Ebbert, Mark T. W. Ebbert   +16 more
doaj   +1 more source

Mapping accuracy of short reads from massively parallel sequencing and the implications for quantitative expression profiling.

PLoS ONE, 2009
BackgroundMassively parallel sequencing offers an enormous potential for expression profiling, in particular for interspecific comparisons. Currently, different platforms for massively parallel sequencing are available, which differ in read length and ...
Nicola Palmieri, Christian Schlötterer
doaj   +1 more source

Deciphering transcriptional plasticity in pancreatic ductal adenocarcinoma reveals alterations in sensory neuron innervation

Molecular Oncology, EarlyView.
Pancreatic sensory neurons innervating healthy and PDAC tissue were retrogradely labeled and profiled by single‐cell RNA sequencing. Tumor‐associated innervation showed a dominant neurofilament‐positive subtype, altered mitochondrial gene signatures, and reduced non‐peptidergic neurons.
Elena Genova, Michele Montrone, Uday Rangaswamy, Francesco Diversi, Irene Schiavo, Denise Ferrarini, Roberta Di Florio, Irene Longo, Michele Coscia, Nicola Zamboni, Giorgia Demontis, Lisa Veghini, Vincenzo Corbo, Remo Sanges, Paul Heppenstall   +14 more
wiley   +1 more source

Data for "3D Printing-Enabled DNA Extraction for Long-Read Genomics" published as ACS Omega 2020, 5, 20817-20824

, 2019
Sizing data is measured in genome mapping nanochannels designed by Bionano Genomics. Concentration data is measured using spectrophotometry to ultimately output DNA amount in a sample.The deposited data files have DNA size measurement critical to ...
Reifenberger, Jeffrey G, Agrawal, Paridhi, Dorfman, Kevin D   +2 more
core   +1 more source