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Pullulan reduces the non-specific amplification of loop-mediated isothermal amplification (LAMP)

Analytical and Bioanalytical Chemistry, 2019
Loop-mediated isothermal amplification (LAMP) has been developed as a versatile method for nucleic acid analysis in many applications. However, non-specific LAMP leading to false-positive outcomes has been observed frequently. To solve this problem, we selected six molecules as the additives for evaluating their effects on the improvement of the LAMP ...
Xueqin, Gao, Bingqi, Sun, Yifu, Guan
openaire   +2 more sources

Enhancing Loop-Mediated Isothermal Amplification through Nonpowered Nanoelectric Preconcentration

Analytical Chemistry
The global threat posed by the COVID-19 pandemic has catalyzed the development of point-of-care (POC) molecular diagnostics. While loop-mediated isothermal amplification (LAMP) stands out as a promising technique among FDA-approved methods, it is occasionally susceptible to a high risk of false positives due to nonspecific amplification of a primer ...
Na Eun Lee   +7 more
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การพัฒนาวิธีการตรวจเนื้อสุกรปนเปื้อนในผลิตภัณฑ์อาหารโดยวิธี loop-mediated isothermal amplification

ได้ทำการพัฒนาการตรวจวิเคราะห์การปนเปื้อนเนื้อสุกรในผลิตภัณฑ์อาหารด้วยวิธี Loop-mediated isothermal amplification (LAMP) และวิธี LAMP-Dot Blotting โดยการออกแบบไพรเมอร์ 4 เส้นคือ Forward Inner Primer (FIP), Backward Inner Primer (BIP), Outer Primer F3 และ Outer Primer B3 ที่จำเพาะยีน cytochrome b และเพิ่มปริมาณดีเอ็นเอสุกรด้วยวิธี LAMP พบว่าสภาวะท ...
openaire   +1 more source

Loop-Mediated Isothermal Amplification-Based Detection of Fusarium graminearum

2012
Molecular biological detection and quantification of fungal DNA targets today relies mainly on the -application of the polymerase chain reaction (PCR). However, this well-recognized technique necessitates the use of highly purified DNA, in a well-equipped lab environment by trained personnel.
openaire   +2 more sources

Loop-Mediated Isothermal Amplification (LAMP)

2020
Alangar Ishwara Bhat, Govind Pratap Rao
openaire   +1 more source

Genome folding through loop extrusion by SMC complexes

Nature Reviews Molecular Cell Biology, 2021
Iain F Davidson, Jan-Michael Peters
exaly  

Progress in multiplex loop-mediated isothermal amplification technology.

Yi chuan = Hereditas, 2015
Loop-mediated isothermal amplification (LAMP) has been widely applied in nucleic acid diagnostics due to its high sensitivity and specificity, high speed and low requirement of equipment. In order to fully leverage these merits, achieve high efficiency and reliability in diagnostics, and expand the applicable fields while keeping low reagent cost ...
Wen-hui, Lin   +3 more
openaire   +1 more source

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