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Loop-mediated isothermal amplification of DNA

open access: yesLoop-mediated isothermal amplification of DNA
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Digital Recombinase Polymerase Amplification, Digital Loop-Mediated Isothermal Amplification, and Digital CRISPR-Cas Assisted Assay: Current Status, Challenges, and Perspectives.

Small, 2023
Digital nucleic acid detection based on microfluidics technology can quantify the initial amount of nucleic acid in the sample with low equipment requirements and simple operations, which can be widely used in clinical and in vitro diagnosis.
Weihong Yin   +6 more
semanticscholar   +1 more source

CRISPR/Cas12a-Assisted Ligation-Initiated Loop-Mediated Isothermal Amplification (CAL-LAMP) for Highly Specific Detection of microRNAs.

Analytical Chemistry, 2021
Loop-mediated isothermal amplification (LAMP) has been increasingly applied in nucleic acid detection for clinical diagnosis and monitoring pathogenic microorganisms due to its isothermal nature and high sensitivity.
Mai Zhang   +4 more
semanticscholar   +1 more source

Recombinase assisted loop-mediated isothermal DNA amplification

The Analyst, 2020
A novel method named RALA which combines the advantages of recombinase and loop-mediated amplification was developed. Fast and sequence-specific detection was achieved by introducing a probe named ProofMan.
Gangyi Chen   +11 more
openaire   +2 more sources

Optimizing the performance of digital loop-mediated isothermal amplification

Analytical Biochemistry, 2021
Digital loop-mediated isothermal amplification (dLAMP) is attractive for the detection of nucleic acid due to its superior characteristics including isothermal amplification, absolute quantification, and single-molecule sensitivity. However, dLAMP suffers from the inaccurate quantification caused by low digital efficiency, which means only part of ...
Yingying, Xue   +4 more
openaire   +2 more sources

Applications of Loop-Mediated Isothermal DNA Amplification

Applied Biochemistry and Biotechnology, 2010
During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six ...
Shijun, Fu   +7 more
openaire   +2 more sources

Hendra virus detection using Loop-Mediated Isothermal Amplification

Journal of Virological Methods, 2012
(Uploaded by Plazi for the Bat Literature Project) No abstract provided.
Adam J, Foord   +2 more
openaire   +2 more sources

Detection of porcine parvovirus by loop-mediated isothermal amplification

Journal of Virological Methods, 2009
Loop-mediated isothermal amplification is a novel method for rapid amplification of DNA. It has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. A loop-mediated isothermal amplification assay was developed for the detection of porcine parvovirus.
Chang-mu, Chen, Shang-jin, Cui
openaire   +2 more sources

Detection of fish nocardiosis by loop-mediated isothermal amplification

Journal of Applied Microbiology, 2006
Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a protocol for detecting Nocardia seriolae which is a causative agent of fish nocardiosis, was designed.A set of four primers, two inner and two outer, were designed ...
T, Itano, H, Kawakami, T, Kono, M, Sakai
openaire   +2 more sources

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