Results 311 to 320 of about 52,140 (330)

Colorimetric strategies applicable for loop-mediated isothermal amplification

Journal of Microbiological Methods
In recent years, loop-mediated isothermal amplification (LAMP) has gained popularity for detecting various pathogen-specific genes due to its superior sensitivity and specificity compared to conventional polymerase chain reaction (PCR). The simplicity and flexibility of naked-eye detection of the amplicon make LAMP an ideal rapid and straightforward ...
Syaidatul Akmal, Saifuddin, Roslina, Rashid, Nurin Jazlina, Nor Azmi, Suharni, Mohamad   +3 more
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Loop-mediated isothermal amplification in disposable polyester-toner microdevices

Analytical Biochemistry, 2017
In recent years, isothermal DNA amplification methods have emerged as an alternative in molecular diagnostics due to its ease of operation. The purpose of using isothermal amplification is to simplify the diagnostics work by i) eliminating heating cycles, ii) reducing equipment costs, and iii) providing rapid and accurate results in laboratories with ...
Kezia Gomes de Oliveira, Juliane Cristina Borba, Alexandre Melo Bailão, Célia Maria de Almeida Soares, Emanuel Carrilho, Gabriela Rodrigues Mendes Duarte   +5 more
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Loop‐Mediated Isothermal Amplification for Detection of Nucleic Acids

Current Protocols in Molecular Biology, 2014
AbstractSequence‐specific isothermal nucleic acid amplification techniques are ideally suited for use in molecular diagnostic applications because they do not require thermal cycling equipment and the reactions are typically fast. One of the most widely cited isothermal techniques is termed loop‐mediated isothermal amplification (LAMP).
Nathan A, Tanner, Thomas C, Evans
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In-Field Diagnostics Using Loop-Mediated Isothermal Amplification

2012
Loop-mediated isothermal amplification (LAMP) is a method for amplification and detection of target organisms which, unlike polymerase chain reaction, does not require thermal cycling. LAMP assays can be developed in the laboratory for subsequent deployment in the field, where the simplicity of isothermal amplification makes LAMP a suitable method for ...
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[Loop-mediated isothermal amplification].

Nihon rinsho. Japanese journal of clinical medicine, 2007
We have developed the loop-mediated isothermal amplification (LAMP) method, an innovative gene amplification technique, which has the potential to become important in genetic testing. This method allows highly efficient amplification by a single enzyme at a constant temperature. It also has features such as high specificity, rapidity, and simplicity of
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Diverse methods of reducing and confirming false-positive results of loop-mediated isothermal amplification assays: A review.

Analytica Chimica Acta, 2023
So-Hee Kim, So-Young Lee, Unji Kim, Se-Wook Oh   +3 more
semanticscholar   +1 more source

Visualization methods for loop mediated isothermal amplification (LAMP) assays

The Analyst
This review explores advancements in LAMP assay visualization techniques, highlighting colorimetric, fluorescence, and nanoparticle-based methods that enhance sensitivity, reliability, and field-deployable nucleic acid detection.
Vinni Thekkudan Novi, Anil Kumar Meher, Abdennour Abbas   +2 more
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Pullulan reduces the non-specific amplification of loop-mediated isothermal amplification (LAMP)

Analytical and Bioanalytical Chemistry, 2019
Loop-mediated isothermal amplification (LAMP) has been developed as a versatile method for nucleic acid analysis in many applications. However, non-specific LAMP leading to false-positive outcomes has been observed frequently. To solve this problem, we selected six molecules as the additives for evaluating their effects on the improvement of the LAMP ...
Xueqin, Gao, Bingqi, Sun, Yifu, Guan
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Enhancing Loop-Mediated Isothermal Amplification through Nonpowered Nanoelectric Preconcentration

Analytical Chemistry
The global threat posed by the COVID-19 pandemic has catalyzed the development of point-of-care (POC) molecular diagnostics. While loop-mediated isothermal amplification (LAMP) stands out as a promising technique among FDA-approved methods, it is occasionally susceptible to a high risk of false positives due to nonspecific amplification of a primer ...
Na Eun Lee, Kang Hyeon Kim, Youngkyu Cho, Jinhwan Kim, Seungmin Kwak, Dohwan Lee, Dae Sung Yoon, Jeong Hoon Lee   +7 more
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การพัฒนาวิธีการตรวจเนื้อสุกรปนเปื้อนในผลิตภัณฑ์อาหารโดยวิธี loop-mediated isothermal amplification

ได้ทำการพัฒนาการตรวจวิเคราะห์การปนเปื้อนเนื้อสุกรในผลิตภัณฑ์อาหารด้วยวิธี Loop-mediated isothermal amplification (LAMP) และวิธี LAMP-Dot Blotting โดยการออกแบบไพรเมอร์ 4 เส้นคือ Forward Inner Primer (FIP), Backward Inner Primer (BIP), Outer Primer F3 และ Outer Primer B3 ที่จำเพาะยีน cytochrome b และเพิ่มปริมาณดีเอ็นเอสุกรด้วยวิธี LAMP พบว่าสภาวะท ...
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