Results 41 to 50 of about 9,592,257 (290)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Power-to-Noise Optimization in the Design of Neural Recording Amplifier Based on Current Scaling, Source Degeneration Resistor, and Current Reuse

Biosensors
This article presents the design of a low-power, low-noise neural signal amplifier for neural recording. The structure reduces the current consumption of the amplifier through current scaling technology and lowers the input-referred noise of the ...
Zhen Wang, Xiao Wang, Guijun Shu, Meng Yin, Shoushuang Huang, Ming Yin   +5 more
doaj   +1 more source

Low-Noise Amplifier Reliability

, 2016
This chapter talks about low-noise amplifier performance degradation subjected to hot electron effect.
Yuan, Jiann Shiun, Jiann-Shiun Yuan
core   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

Low noise figure C+L-band erbium-droped fiber amplifier

Dianxin kexue, 2017
A new configuration of the low noise figure C+L-band erbium-doped fiber amplifier was proposed and demonstrated.In the proposed configuration,a preamplifier was used to reduce the noise figure,and an improved double-pass configuration with a fiber bragg ...
Hangdong ZHANG, Li ZHAN
doaj   +2 more sources

Design of two-stage low noise amplifier [PDF]

, 2015
DESIGN OF A MICROWAVE TWO-STAGE LOW NOISE AMPLIFIER AT 17GHz BY ABHINAV PACHIKANTI MASTER OF SCIENCE IN ELECTICAL ENGINEERING The goal of the project is to design a low noise amplifier to meet the specific noise figure less than 2dB and Gain more than be
Pachikanti, Abhinav
core  

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Low-loss meta-atom for improved resonance response

AIP Advances, 2012
Measurements of a meta-atom integrated with a low noise amplifier into the split-ring resonator are presented. A comparison is made between baseline meta-atoms and one integrated with a GaAs low noise amplifier.
Derrick Langley, Ronald A. Coutu Jr., Peter J. Collins   +2 more
doaj   +1 more source