Results 91 to 100 of about 49,910 (293)

BRET-based reporter conjugated luminescent nanoparticles for improved bioluminescence imaging [PDF]

, 2023
The emergence of bright bioluminescent luciferase reporters has enabled versatilebioimaging applications in vitro and in vivo . However, conventional luciferases such as nanoluciferase are sub-optimal reporters for bioluminescence imaging (BLI) in vivo ...
Tahir, Mahum
core  

CRISPR-Mediated Tagging of Endogenous Proteins with a Luminescent Peptide

ACS Chemical Biology, 2017
Intracellular signaling pathways are mediated by changes in protein abundance and post-translational modifications. A common approach for investigating signaling mechanisms and the effects induced by synthetic compounds is through overexpression of recombinant reporter genes.
Marie K. Schwinn, Thomas Machleidt, Kris Zimmerman, Christopher T. Eggers, Andrew S. Dixon, Robin Hurst, Mary P. Hall, Lance P. Encell, Brock F. Binkowski, Keith V. Wood   +9 more
openaire   +2 more sources

ELABELA Targets Mitochondria to Modulate Heart Development

Advanced Science, EarlyView.
The role of peptide ELABELA (ELA) in cardiomyocyte apoptosis and congenital heart disease (CHD) is unclear. ELA deficiency caused cardiomyocyte apoptosis and CHD. A novel ELA‐APJ‐AKT‐BCL2/BAX axis in regulating mitochondrial function and contributing to CHD pathogenesis was established.
Jian Wang, Qingjie Wang, Zhikang Xu, Shuang Zhou, Yue Zhou, Junjie Yang, Lingfeng Tong, Zhuo Meng, Mei Yang, Wen Zhao, Tie Yang, Hualin Wang, Jun Zhang, Rubin Tan, Lei Wang, Yuqiang Huang, Bin Zhou, Sun Chen, Bing Zhang, Jinxiang Yuan, Jianyuan Zhao, Alex F. Chen, Kun Sun   +22 more
wiley   +1 more source

Sensing Proteins with Luminescent Silica Nanoparticles

, 2016
Nanometer sized silica nanoparticles (SiO2-NP) were prepared in water and loaded with two organic compounds, namely perylene and 1,6-diphenyl-1,3,5-hexatriene, which have well-defined and known fluorescence properties. The size of void and dye-doped SiO2-
Loredana Latterini (1494481), Matteo Amelia (1890997)   +1 more
core   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Ankerhold, Richard, Richard Ankerhold, Drummen, Gregor P. C., Gregor P. C. Drummen, Hellen C. Ishikawa-Ankerhold, Ishikawa-Ankerhold, Hellen C.   +5 more
core   +1 more source

Probing Temperature- and pH-Dependent Binding between Quantum Dots and Bovine Serum Albumin by Fluorescence Correlation Spectroscopy

Nanomaterials, 2017
Luminescent quantum dots (QDs) with unique optical properties have potential applications in bio-imaging. The interaction between QDs and bio-molecules is important to the biological effect of QDs in vivo.
Zonghua Wang, Qiyan Zhao, Menghua Cui, Shichao Pang, Jingfang Wang, Ying Liu, Liming Xie   +6 more
doaj   +1 more source

QBP1 Peptide as a Potential Anti‐Amyloidogenic Therapy for Type 2 Diabetes: An In Vitro Study

Advanced Science, EarlyView.
The anti‐amyloidogenic peptide QBP1 effectively halts human islet amyloid polypeptide (hIAPP) aggregation, preventing the formation of toxic β‐structured intermediates. Through a combination of biophysical assays, molecular dynamics, and cell‐based studies, QBP1 is shown to preserve β‐cell viability and metabolic homeostasis, positioning it as a ...
María M. Tejero‐Ojeda, Ada Bernaus Vives, Michał Wojciechowski, Dinh Quoc Huy Pham, Mateusz Chwastyk, Mario Vallejo, Anna Novials, Douglas V. Laurents, Mariano Carrión‐Vázquez   +8 more
wiley   +1 more source

A Luminescent Cyclometalated Iridium(III) Complex Accumulates in Mitochondria and Induces Mitochondrial Shortening by Conjugation to Specific Protein Targets

, 2012
We report the cellular properties of a luminescent cyclometalated iridium(III) complex, [Ir(pq)2(phen-ITC)](PF6) (Ir-ITC; Hpq=2-phenylquinoline, phen-ITC=5-isothiocyanate-1,10-phenanthroline), that efficiently and specifically labels mitochondria in ...
Wang, Baojiang, Yun Wah Lam, Lam, Yun Wah, Cheng, Jinping, Jinping Cheng, Hongjuan Dong, Bao Zhan, Shuk Han Cheng, Dong, Hongjuan, Tianfeng Tan, Kenneth Kam‐Wing Lo, Cheng, Shuk Han, Lo, Kenneth Kam Wing, Baojiang Wang, Liang, Yimin, Yimin Liang, Zhan, Bao, Tan, Tianfeng   +17 more
core   +1 more source

Engineered Mesenchymal Stem Cell–NK Cell Complexes for Spatially Targeted and Functionally Revitalized Cancer Immunotherapy

Advanced Science, EarlyView.
IL‐15‐engineered stem cell–NK cell complexes, assembled via bioorthogonal chemistry, enable effective lung cancer immunotherapy. Abstract Natural killer (NK) cells represent a powerful immunotherapeutic strategy due to their intrinsic cytotoxicity and ability to target tumor cells independently of antigen presentation.
Qian Zhang, Bo Yin, Munaiwaier Sabier, Youlan Yang, Mengling Wu, Zhouping Zhao, Xuanzhi Luo, Yuehong Zhong, Xiuming Zhu, Jie Zhang, Jing Wang, Kai Chen, Fei Ruan, Wei Zhang, Zhimin Lu, Jiong Wang   +15 more
wiley   +1 more source

Methods for nanoparticle labeling of ricin and effect on toxicity

, 2009
The unique optical properties associated with nanostructured materials that support the excitation of surface plasmons offer many new opportunities for the enhanced optical investigation of biological materials that pose a security threat. In particular,
Jun Yu, Graham, Duncan, Christopher D. Lindsay, Nativo, Paola, Alastair W. Wark, Duncan Graham, Wark, Alastair, Paola Nativo, Yu, Jun, Lindsay, C. D.   +9 more
core   +1 more source