Results 101 to 110 of about 49,910 (293)

Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity

Advanced Science, EarlyView.
This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley   +1 more source

Luminescent Pyrimidine Hydrazide Oligomers with Peptide Affinity

, 2006
The modular synthesis of pyrimidine oligohydrazides and their peptide binding ability are reported. Ethylene glycol substituents ensure water solubility of the compounds.
König, B., Li, X., Miltschitzky, Stefan
core   +1 more source

A Fluorescent Reporter Virus Toolkit for Interrogating Enterovirus Biology and Host Interactions

Viruses
Enteroviruses are a group of highly prevalent human pathogens responsible for a wide range of illnesses, ranging from common cold symptoms to life-threatening diseases.
Mireya Martínez-Pérez, Sebastian Velandia-Álvarez, Cristina Vidal-Verdú, Beatriz Álvarez-Rodríguez, Ron Geller   +4 more
doaj   +1 more source

Calcitriol Reverses Induced Expression of Efflux Proteins and Potentiates Cytotoxic Activity of Gemcitabine in Capan-2 Pancreatic Cancer Cells

Journal of Pharmacy & Pharmaceutical Sciences, 2017
Purpose. Efflux and influx proteins play a major role in chemo-resistance by affecting the net cellular uptake of anti-cancer drugs. Hence, alteration of the efflux and influx protein expression may result in variations of chemotherapeutics uptake and ...
Hamed Gilzad-Kohan, Shabnam Sani, Mehdi Boroujerdi   +2 more
doaj   +1 more source

LMO7 Suppresses Tumor‐Associated Macrophage Phagocytosis of Tumor Cells Through Degradation of LRP1

Advanced Science, EarlyView.
LMO7 in tumor‐associated macrophages suppresses phagocytosis of tumor cells and limits cytotoxic T lymphocytes infiltration, fostering tumor progression. Mechanistically, LMO7 mediates the ubiquitination and degradation of the phagocytic receptor LRP1, impairing its ability to engulf tumor cells and driving macrophages toward an antitumor phenotype ...
Mengkai Li, Tao Wang, Yuwen Zhong, Zhiming Wang, Wei Li, Zejian Wang, Xinyi Yang, Yu Qiao, Jianfeng Xiang, Wei Gu, Zishu Wang, Lei Sun, Feng Qian   +12 more
wiley   +1 more source

Natural Products Inspired Scaffold Diversification Leads to Unnatural Molecular Warhead and Covalent Strategy to Modulating Protein Function through Electrophilic Bromine Transfer

Advanced Science, EarlyView.
We report a new thiolate‐reactive α,α‐gem‐dibromo lactam warhead that activates transcription factor Nrf2 and demonstrates anti‐inflammatory activities, which have implications in cancer, neurodegeneration, and cardiovascular diseases. RNA‐seq illuminated detailed transcriptional profiles, and chemical reactions with cysteine‐containing compounds ...
Beau R. Brummel, Sofia Kokkaliari, Qiwen Gao, Jinjing Chen, Larissa Costa de Almeida, Srinivasarao Tenneti, Derek A. Leas, Eli Levit, Lewis S. Alexander, Khalil A. Abboud, Pingrong Wei, Michael D. Cameron, James R. Rocca, Ranjala Ratnayake, Donna D. Zhang, Hendrik Luesch, Robert W. Huigens III   +16 more
wiley   +1 more source

Paper-based biodetection using luminescent nanoparticles

, 2016
Point-of-care and in-field technologies for rapid, sensitive and selective detection of molecular biomarkers have attracted much interest. Rugged bioassay technology capable of fast detection of markers for pathogens and genetic diseases would in ...
Ju, Qiang, Noor, M. Omair, Krull, Ulrich J.   +2 more
core   +1 more source

Fibrates Inhibit PLTP‐induced M2 Macrophage Infiltration and Increase the Sensitivity of Hepatocellular Carcinoma to ICIs

Advanced Science, EarlyView.
Phospholipid transfer protein(PLTP) plays a critical role in forming a complex with kinase A (AURKA) and P65. This interaction facilitates phosphorylation of P65 at Ser536, leading to the activation of the NF‐κB signaling pathway. Ultimately, this leads to the upregulation of downstream cytokines, including IL‐6, IL‐8, and CSF‐1, which promotes M2 ...
Xinyue Liang, Yaning Li, Jianxun Cai, Lisi Luo, Pengfei Yang, Yutong Chen, Jiancong Zhou, Yan Zeng, Jiaping Yu, Weiyu Zhang, Wenzheng Pang, Yufang Li, Chunhua Wen, Jian Li, Linjuan Zeng   +14 more
wiley   +1 more source

The kinase activities of GST fusion proteins were measured in vitro.

, 2013
A. Phosphorylation activities of GST fusion proteins containing full-length Stk (using plasmid pQG77), Stk1–263 (pQG78) or Stk40–263(pQG79) were measured with a Kinase-Glo™ luminescent kinase assay in vitro at 32°C for 4 h. B.
Jianping Wang (215292), Min Li (12799), Qian Liu (135614), Jiajia Fan (343396), Xing Wang (154377), Amer E. Villaruz (343399), Chen Niu (343397), Decheng Wang (343398), Michael Otto (40636), Qian Gao (146991)   +9 more
core   +1 more source

Quantitative analysis of electroporation-mediated intracellular delivery via bioorthogonal luminescent reaction

Communications Chemistry
Efficient intracellular delivery is crucial for biotherapeutics, such as proteins, oligonucleotides, and CRISPR/Cas9 gene-editing systems, to achieve their efficacy. Despite the great efforts of developing new intracellular delivery carriers, the lack of
Shiqi Wang, Mariia V. Shcherbii, Sami-Pekka Hirvonen, Gudrun Silvennoinen, Mirkka Sarparanta, Hélder A. Santos   +5 more
doaj   +1 more source