Results 21 to 30 of about 49,048 (258)

A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis

BMC Plant Biology, 2009
Background Ubiquitination is mediated by the sequential action of at least three enzymes: the E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme) and E3 (ubiquitin ligase) proteins. Polyubiquitination of target proteins is also implicated
Seki Motoaki, Nozawa Akira, Takahashi Hirotaka, Shinozaki Kazuo, Endo Yaeta, Sawasaki Tatsuya   +5 more
doaj   +1 more source

Design of an artificial phage-display library based on a new scaffold improved for average stability of the randomized proteins

Scientific Reports, 2023
Scaffold-based protein libraries are designed to be both diverse and rich in functional/folded proteins. However, introducing an extended diversity while preserving stability of the initial scaffold remains a challenge.
M. Gomes, A. Fleck, A. Degaugue, F. Gourmelon, C. Léger, M. Aumont-Nicaise, A. Mesneau, H. Jean-Jacques, G. Hassaine, A. Urvoas, P. Minard, M. Valerio-Lepiniec   +11 more
doaj   +1 more source

Control of actin polymerization via reactive oxygen species generation using light or radiation

Frontiers in Cell and Developmental Biology, 2022
Actin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility,
Tetsuya Ishimoto, Tetsuya Ishimoto, Hisashi Mori, Hisashi Mori, Hisashi Mori   +4 more
doaj   +1 more source

Luminescent Metal Nanoclusters for Potential Chemosensor Applications

Chemosensors, 2017
Studies of metal nanocluster (M-NCs)-based sensors for specific analyte detection have achieved significant progress in recent decades. Ultra-small-size (<2 nm) M-NCs consist of several to a few hundred metal atoms and exhibit extraordinary physical ...
Muthaiah Shellaiah, Kien Wen Sun
doaj   +1 more source

Bioluminescent monitoring of recombinant lactic acid bacteria and their products

mBio, 2023
Biotherapeutic strategies to promote health, including the application of engineered microbes to deliver therapeutic molecules, hold strong promise. However, without precision tools to detect therapeutic microbes and their products, we are hampered in ...
In Young Choi, Jee-Hwan Oh, Zhiying Wang, Jan-Peter van Pijkeren   +3 more
doaj   +1 more source

Interglobular Diffusion of an Energy Donor in Triplet-Triplet Energy Transfer in Proteins

Journal of Spectroscopy, 2013
The triplet-triplet energy transfer between polar molecules of luminescent probe (eosin) as an energy donor and nonpolar molecules of energy acceptor (anthracene) is studied. Both the donor and the acceptor are bound to human serum albumin by noncovalent
Andrey G. Melnikov, Alexander B. Pravdin, Vyacheslav I. Kochubey, Anna V. Kuptsova, Gennady V. Melnikov   +4 more
doaj   +1 more source

A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae

Microbial Cell, 2022
Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs.
Katrin Hieronimus, Tabea Donauer, Jonas Klein, Bastian Hinkel, Julia Vanessa Spänle, Anna Probst, Justus Niemeyer, Salina Kibrom, Anna Maria Kiefer, Luzia Schneider, Britta Husemann, Eileen Bischoff, Sophie Möhring, Nicolas Bayer, Dorothée Klein, Adrian Engels, Benjamin Gustav Ziehmer, Julian Stieß, Pavlo Moroka, Michael Schroda, Marcel Deponte   +20 more
doaj   +1 more source

New Cationic fac-[Re(CO)3(deeb)B2]+ Complex, Where B2 Is a Benzimidazole Derivative, as a Potential New Luminescent Dye for Proteins Separated by SDS-PAGE

Frontiers in Chemistry, 2021
Sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE) can be used to separate proteins based mainly on their size such as in denaturing gels.
Alexander Carreño, Manuel Gacitúa, Eduardo Solis-Céspedes, Eduardo Solis-Céspedes, Dayán Páez-Hernández, Wesley B. Swords, Gerald J. Meyer, Marcelo D. Preite, Ivonne Chávez, Andrés Vega, Andrés Vega, Juan A. Fuentes   +11 more
doaj   +1 more source

Far-red pentamethine cyanine dyes as fluorescent probes for the detection of serum albumins [PDF]

Royal Society Open Science, 2020
Benzothiazole based cyanine dyes with bridged groups in the pentamethine chain were studied as potential far-red fluorescent probes for protein detection.
D. Aristova, G. Volynets, S. Chernii, M. Losytskyy, A. Balanda, Yu. Slominskii, A. Mokhir, S. Yarmoluk, V. Kovalska   +8 more
doaj   +1 more source

Generation of Reporter-Expressing New World Arenaviruses: A Systematic Comparison

Viruses, 2022
Replication-competent reporter-expressing viruses are crucial tools in molecular virology with applications that range from antiviral screening to live-cell imaging of protein spatiotemporal dynamics.
Lucie Fénéant, Anne Leske, Karla Günther, Allison Groseth   +3 more
doaj   +1 more source