Results 21 to 30 of about 1,644,009 (295)

On the selection and design of proteins and peptide derivatives for the production of photoluminescent, red-emitting gold quantum clusters [PDF]

open access: yes, 2013
Novel pathways of the synthesis of photoluminescent gold quantum clusters (AuQCs) using biomolecules as reactants provide biocompatible products for biological imaging techniques.
Naszályi Nagy, Lívia   +20 more
core   +1 more source

Five colour variants of bright luminescent protein for real-time multicolour bioimaging

open access: yesNature Communications, 2016
The use of luminescence, rather than fluorescence, for imaging is advantageous in situations where laser illumination should be avoided. Here the authors make five colour variants of bright luminescent proteins based on FRET between NLuc and fluorescent ...
Kazushi Suzuki   +7 more
doaj   +1 more source

A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis

open access: yesBMC Plant Biology, 2009
Background Ubiquitination is mediated by the sequential action of at least three enzymes: the E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme) and E3 (ubiquitin ligase) proteins. Polyubiquitination of target proteins is also implicated
Seki Motoaki   +5 more
doaj   +1 more source

Design of an artificial phage-display library based on a new scaffold improved for average stability of the randomized proteins

open access: yesScientific Reports, 2023
Scaffold-based protein libraries are designed to be both diverse and rich in functional/folded proteins. However, introducing an extended diversity while preserving stability of the initial scaffold remains a challenge.
M. Gomes   +11 more
doaj   +1 more source

Control of actin polymerization via reactive oxygen species generation using light or radiation

open access: yesFrontiers in Cell and Developmental Biology, 2022
Actin is one of the most prevalent proteins in cells, and its amino acid sequence is remarkably conserved from protozoa to humans. The polymerization-depolymerization cycle of actin immediately below the plasma membrane regulates cell function, motility,
Tetsuya Ishimoto   +4 more
doaj   +1 more source

Luminescent Metal Nanoclusters for Potential Chemosensor Applications

open access: yesChemosensors, 2017
Studies of metal nanocluster (M-NCs)-based sensors for specific analyte detection have achieved significant progress in recent decades. Ultra-small-size (<2 nm) M-NCs consist of several to a few hundred metal atoms and exhibit extraordinary physical ...
Muthaiah Shellaiah, Kien Wen Sun
doaj   +1 more source

Bioluminescent monitoring of recombinant lactic acid bacteria and their products

open access: yesmBio, 2023
Biotherapeutic strategies to promote health, including the application of engineered microbes to deliver therapeutic molecules, hold strong promise. However, without precision tools to detect therapeutic microbes and their products, we are hampered in ...
In Young Choi   +3 more
doaj   +1 more source

Interglobular Diffusion of an Energy Donor in Triplet-Triplet Energy Transfer in Proteins

open access: yesJournal of Spectroscopy, 2013
The triplet-triplet energy transfer between polar molecules of luminescent probe (eosin) as an energy donor and nonpolar molecules of energy acceptor (anthracene) is studied. Both the donor and the acceptor are bound to human serum albumin by noncovalent
Andrey G. Melnikov   +4 more
doaj   +1 more source

A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae

open access: yesMicrobial Cell, 2022
Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs.
Katrin Hieronimus   +20 more
doaj   +1 more source

Preliminary Investigation on the Physiology and Ecology of Luminescence in the Copepod, Metridia lucens [PDF]

open access: yes, 1961
1. Skin glands believed to be the source of luminescence were found on the anterior portion of the head, on the last thoracic segment, and on the posterior margins of each segment of the abdomen. 2. The maximum intensity of the luminescent flash was 1.2
Conover, Robert J.   +4 more
core   +1 more source

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