Results 31 to 40 of about 78,007 (304)

Design of an artificial phage-display library based on a new scaffold improved for average stability of the randomized proteins

Scientific Reports, 2023
Scaffold-based protein libraries are designed to be both diverse and rich in functional/folded proteins. However, introducing an extended diversity while preserving stability of the initial scaffold remains a challenge.
M. Gomes, A. Fleck, A. Degaugue, F. Gourmelon, C. Léger, M. Aumont-Nicaise, A. Mesneau, H. Jean-Jacques, G. Hassaine, A. Urvoas, P. Minard, M. Valerio-Lepiniec   +11 more
doaj   +1 more source

Bioluminescent monitoring of recombinant lactic acid bacteria and their products

mBio, 2023
Biotherapeutic strategies to promote health, including the application of engineered microbes to deliver therapeutic molecules, hold strong promise. However, without precision tools to detect therapeutic microbes and their products, we are hampered in ...
In Young Choi, Jee-Hwan Oh, Zhiying Wang, Jan-Peter van Pijkeren   +3 more
doaj   +1 more source

Advanced Fluorescence Microscopy Techniques-FRAP, FLIP, FLAP, FRET and FLIM [PDF]

, 2012
Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity.
Abbe, Abbe, Ai, Airy, Alivisatos, Alivisatos, Ando, Aoki, Auksorius, Auzel, Auzel, Axelrod, Axelrod, Bacskai, Bader, Bader, Balse, Barroso, Bastiaens, Bates, Beaudouin, Beaurepaire, Becker, Benninger, Berezin, Berney, Betzig, Bianchini, Biju, Binnemans, Borst, Brakemann, Bretschneider, Bunt, Campagnola, Campagnola, Campbell, Cantor, Carrero, Chalfie, Chalfie, Chatterjee, Chen, Chen, Clapp, Clapp, Clapp, Cole, Coons, Dahan, Dedecker, Del Pozo, Deng, Denk, Diaspro, Diaspro, Dickson, Digman, Drobizhev, Drummen, Dundr, Dundr, Dunn, Dunn, Dunn, Durr, Dyba, Ehrlicher, Einstein, Elangovan, Elder, Ellenberg, Elsner, Feige, Fereidouni, Festenstein, Frommer, Förster, Förster, Gamelin, Garcia-Mira, Garcia-Parajo, Gerlich, Giese, Giordano, Goodson, Gopich, Gordon, Gregor P. C. Drummen, Grotjohann, Gu, Gunnlaugsson, Gurskaya, Gustafsson, Göppert-Mayer, Ha, Haugland, Heimstädt, Hellen C. Ishikawa-Ankerhold, Hemmila, Hiller, Hitakomate, Hofkens, Hofmann, Houtsmuller, Houtsmuller, Howell, Hu, Hu, Huang, Ishihama, Jacobson, Jares-Erijman, Jiang, Johannes, Johnson, Jonkman, Jose, Jovin, Jovin, Kaiser, Kambara, Kapustina, Karasawa, Kardash, Kasha, Kawashima, Kenworthy, Kerppola, Kerppola, Kerppola, Kerppola, Kimura, Kinoshita, Klonis, Kner, Knoblauch, Konig, Koppel, Koster, Kota, Koushik, Kremers, Kremers, Kuningas, Kuningas, Kuningas, Laforge, Lakowicz, Lakowicz, Larson, Lee, Lichtman, Liebman, Lippincott-Schwartz, Liu, Llères, Loura, Lukyanov, Lundin, Mansfield, Mathew-Fenn, Mattheyses, Matz, Medintz, Mills, Mitchell, Miyawaki, Miyawaki, Moerner, Moneron, Monetta, Mueller, Mueller, Nagai, Nehls, Ormö, Paddock, Parsons, Patterson, Patterson, Patterson, Pawley, Pawley, Persechini, Peters, Phair, Phair, Phillips, Piston, Piston, Planck, Pockwinse, Politz, Poo, Puliti, Rajaram, Rantanen, Reinhard, Reits, Reymann, Richard Ankerhold, Richards, Riven, Rizzo, Rizzo, Robinson, Roduner, Rotblat, Roy, Rust, Sakon, Sbalzarini, Schawlow, Schönle, Seabrooke, Seah, Selvin, Shaner, Shav-Tal, Shaw, Shchyolkina, Shimomura, Shimomura, Shroff, Shu, Shyu, Smith, So, Solon, Soukka, Soumpasis, Sprague, Sprague, Stasevich, Stelzer, Stepanenko, Stokes, Stryer, Stryer, Subach, Sun, Suyver, Swaminathan, Swift, Sönnichsen, Tagawa, Taylor, Thaler, Tian, Tian, Tinnefeld, Tisler, Tocanne, Tomosugi, Tripathi, Tsien, Tsien, Uphoff, Valentin, van Driel, Van Munster, Van Munster, van Munster, van Rheenen, van Royen, van Royen, van Royen, Varghese, Venetta, Vogelsang, von Wichert, Vosch, Wallrabe, Wang, Wang, Wang, Wang, Wang, Wang, Welch, White, Williams, Willig, Wouters, Wouters, Wouters, Wouters, Wright, Xia, Yang, Youvan, Zhang, Zhang, Zimmer, Zipfel   +295 more
core   +2 more sources

New compound sets identified from high throughput phenotypic screening against three kinetoplastid parasites:an open resource [PDF]

, 2015
Using whole-cell phenotypic assays, the GlaxoSmithKline high-throughput screening (HTS) diversity set of 1.8 million compounds was screened against the three kinetoplastids most relevant to human disease, i.e.
Alonso-Padilla, Julio, Alvarez, Emilio, Bardera, Ana I., Barroso, Vanessa, Brown, James R., Cantizani, Juan, Colmenarejo, Gonzalo, Cotillo, Ignacio, de Dios-Anton, Francisco, Drewry, David H., Fiandor, Jose M., Gray, David W., Julio Martin, J., Kessler, Albane, Kumar, Vinod, Navarro, Miguel, Peña, Imanol, Pilar Manzano, M., Rodriguez, Ana, Roquero, Irene, Sherstnev, Alexander   +20 more
core   +3 more sources

Interglobular Diffusion of an Energy Donor in Triplet-Triplet Energy Transfer in Proteins

Journal of Spectroscopy, 2013
The triplet-triplet energy transfer between polar molecules of luminescent probe (eosin) as an energy donor and nonpolar molecules of energy acceptor (anthracene) is studied. Both the donor and the acceptor are bound to human serum albumin by noncovalent
Andrey G. Melnikov, Alexander B. Pravdin, Vyacheslav I. Kochubey, Anna V. Kuptsova, Gennady V. Melnikov   +4 more
doaj   +1 more source

A Modular Cloning Toolkit for the production of recombinant proteins in Leishmania tarentolae

Microbial Cell, 2022
Modular Cloning (MoClo) is based on libraries of standardized genetic parts that can be directionally assembled via Golden Gate cloning in one-pot reactions into transcription units and multigene constructs.
Katrin Hieronimus, Tabea Donauer, Jonas Klein, Bastian Hinkel, Julia Vanessa Spänle, Anna Probst, Justus Niemeyer, Salina Kibrom, Anna Maria Kiefer, Luzia Schneider, Britta Husemann, Eileen Bischoff, Sophie Möhring, Nicolas Bayer, Dorothée Klein, Adrian Engels, Benjamin Gustav Ziehmer, Julian Stieß, Pavlo Moroka, Michael Schroda, Marcel Deponte   +20 more
doaj   +1 more source

Synthesis and Functionalization of Monodisperse Near-ultraviolet and Visible Excitable Multifunctional Eu3+, Bi3+:REVO4 Nanophosphors for Bioimaging and Biosensing Applications [PDF]

, 2016
Near-ultraviolet and visible excitable Eu- and Bi-doped NPs based on rare earth vanadates (REVO4, RE = Y, Gd) have been synthesized by a facile route from appropriate RE precursors, europium and bismuth nitrate, and sodium orthovanadate, by homogeneous ...
Ashraf, Sumaira, Carrillo Carrión, Carolina, Escudero Belmonte, Alberto, Hartmann,Raimo, Núñez Álvarez, Nuria Ofelia, Ocaña Jurado, Manuel, Parak, Wolfgang J., Zyuzin, Mikhail V.   +7 more
core   +1 more source

Far-red pentamethine cyanine dyes as fluorescent probes for the detection of serum albumins [PDF]

Royal Society Open Science, 2020
Benzothiazole based cyanine dyes with bridged groups in the pentamethine chain were studied as potential far-red fluorescent probes for protein detection.
D. Aristova, G. Volynets, S. Chernii, M. Losytskyy, A. Balanda, Yu. Slominskii, A. Mokhir, S. Yarmoluk, V. Kovalska   +8 more
doaj   +1 more source

Soft X-ray induced oxidation on acrylic acid grafted luminescent silicon quantum dots in ultrahigh vacuum [PDF]

, 2011
Water soluble acrylic acid grafted luminescent silicon quantum dots (Si-QDs) were prepared by a simplified method. The resulting Si-QDs dissolved in water and showed stable strong luminescence with peaks at 436 and 604?nm.
Alsharif, Bagolini, Biju, Boukai, Burg, Canham, Carter, Chao, Chao, Chao, Denecke, Dixon, Franchina, Fujioka, Gaponenko, Hadjadj, Hochbaum, Ishii, Itoh, Komuro, Lehner, Li, Li, Li, Li, Lie, Lin, Liu, Michalet, Moore, Nayfeh, Osipov, Park, Polisski, Puniredd, Rostron, Sato, Sham, Sharp, Sieval, Tamura, Thompson, Tilley, Uhlir, Wang, Warner, Wolkin, Yoshigoe, Šiller   +48 more
core   +1 more source

Generation of Reporter-Expressing New World Arenaviruses: A Systematic Comparison

Viruses, 2022
Replication-competent reporter-expressing viruses are crucial tools in molecular virology with applications that range from antiviral screening to live-cell imaging of protein spatiotemporal dynamics.
Lucie Fénéant, Anne Leske, Karla Günther, Allison Groseth   +3 more
doaj   +1 more source