Crystal structure and catalytic mechanism of PL35 family glycosaminoglycan lyases with an ultrabroad substrate spectrum [PDF]
eLifeRecently, a new class of glycosaminoglycan (GAG) lyases (GAGases) belonging to PL35 family has been discovered with an ultrabroad substrate spectrum that can degrade three types of uronic acid-containing GAGs (hyaluronic acid, chondroitin sulfate and ...
Lin Wei +11 more
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A comparative study of the efficacy of alginate lyases in the presence of metal ions elevated in the cystic fibrosis lung milieu [PDF]
Biochemistry and Biophysics ReportsPseudomonas aeruginosa, a common cause of morbidity in cystic fibrosis, chronically infects the patient's lungs by forming an alginate-containing biofilm.
Neetu, T.N.C. Ramya
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Insights into the mechanism of substrate specificity in a novel PL15_3 subfamily oligo-alginate lyase VBAly15A [PDF]
Applied and Environmental MicrobiologyAlginate is a major component of brown algae cell walls and can be degraded via β-elimination by alginate lyases. These enzymes are classified into polysaccharide lyases and oligo-alginate lyases (Oals), with Oals mainly represented by the PL15 and PL17 ...
Yongqi Tang +4 more
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Recent Advances in Alginate Lyase Engineering for Efficient Conversion of Alginate to Value‐Added Products [PDF]
Microbial BiotechnologyAlginate lyases depolymerize alginate and generate alginate oligosaccharides (AOS) and eventually 4‐deoxy‐L‐erythro‐5‐hexoseulose uronate (DEH), a monosaccharide.
Hyo Jeong Shin +5 more
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Unraveling the molecular mechanism of polysaccharide lyases for efficient alginate degradation [PDF]
Nature CommunicationsAlginate lyases (ALs) catalyze the depolymerization of brown macroalgae alginates, widely used naturally occurring polysaccharides. Their molecular reaction mechanism remains elusive due to the lack of catalytically competent Michaelis-Menten-like ...
José Pablo Rivas-Fernández +12 more
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Lyase to live by: Sphingosine phosphate lyase as a therapeutic target [PDF]
Expert Opinion on Therapeutic Targets, 2009 Sphingosine 1-phosphate (S1P) is a bioactive lipid that regulates cell proliferation, survival and migration and plays an essential role in angiogenesis and lymphocyte trafficking. S1P levels in the circulation and tissues are tightly regulated for proper cell functioning, and dysregulation of this system may contribute to the pathophysiology of ...
Ashok Kumar, Julie D. Saba
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Marine Drugs, 2021 Alginate, the most abundant polysaccharides of brown algae, consists of various proportions of uronic acid epimers α-L-guluronic acid (G) and β-D-mannuronic acid (M).
Shu-Kun Gao +7 more
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Bacterial alginate metabolism: an important pathway for bioconversion of brown algae
Biotechnology for Biofuels, 2021 Brown macroalgae have attracted great attention as an alternative feedstock for biorefining. Although direct conversion of ethanol from alginates (major components of brown macroalgae cell walls) is not amenable for industrial production, significant ...
Lanzeng Zhang +4 more
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Argininosuccinate lyase deficiency [PDF]
Genetics in Medicine, 2012 The urea cycle consists of six consecutive enzymatic reactions that convert waste nitrogen into urea. Deficiencies of any of these enzymes of the cycle result in urea cycle disorders (UCDs), a group of inborn errors of hepatic metabolism that often result in life-threatening hyperammonemia. Argininosuccinate lyase (ASL) catalyzes the fourth reaction in
Brendan Lee +3 more
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The Hydroxynitrile Lyase from Almond [PDF]
Structure, 2001 Cyanogenesis is a defense process of several thousand plant species. Hydroxynitrile lyase, a key enzyme of this process, cleaves a cyanohydrin into hydrocyanic acid and the corresponding aldehyde or ketone. The reverse reaction constitutes an important tool in biocatalysis.
Ingrid Dreveny +4 more
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