Results 1 to 10 of about 1,405,084 (401)

Differential Requirement for the Cell Wall Integrity Sensor Wsc1p in Diploids Versus Haploids

open access: yesJournal of Fungi, 2021
The primary role of the Cell Wall Integrity Pathway (CWI) in Saccharomyces cerevisiae is monitoring the state of the cell wall in response to general life cycle stresses (growth and mating) and imposed stresses (temperature changes and chemicals). Of the
Allison E. Hall   +2 more
doaj   +1 more source

WhyD tailors surface polymers to prevent premature bacteriolysis and direct cell elongation in Streptococcus pneumoniae

open access: yeseLife, 2022
Penicillin and related antibiotics disrupt cell wall synthesis in bacteria causing the downstream misactivation of cell wall hydrolases called autolysins to induce cell lysis.
Josué Flores-Kim   +3 more
doaj   +1 more source

Current State of Method Development for PCR Sample Peparations

open access: yesВестник войск РХБ защиты, 2021
The sensitivity, specificity and reproducibility of molecular genetic methods of analysis largely depend on the quality of the preliminary preparation of the analyzed samples.
M. S. Tikhvinskiy   +5 more
doaj   +1 more source

Interference of the Zika Virus E-Protein With the Membrane Attack Complex of the Complement System

open access: yesFrontiers in Immunology, 2020
The complement system has developed different strategies to clear infections by several effector mechanisms, such as opsonization, which supports phagocytosis, attracting immune cells by C3 and C5 cleavage products, or direct killing of pathogens by the ...
Zahra Malekshahi   +5 more
doaj   +1 more source

The transcriptional regulator CtrA controls gene expression in Alphaproteobacteria phages: Evidence for a lytic deferment pathway

open access: yesFrontiers in Microbiology, 2022
Pilitropic and flagellotropic phages adsorb to bacterial pili and flagella. These phages have long been used to investigate multiple aspects of bacterial physiology, such as the cell cycle control in the Caulobacterales. Targeting cellular appendages for
Elia Mascolo   +8 more
doaj   +1 more source

Image-based dynamic phenotyping reveals genetic determinants of filamentation-mediated beta-lactam tolerance [PDF]

open access: yes, 2020
Antibiotic tolerance characterized by slow killing of bacteria in response to a drug can lead to treatment failure and promote the emergence of resistance.
Camacho, Rafael   +8 more
core   +2 more sources

Genes Controlling 2-deoxyglucose Induced Lysis and Formation of Reactive Oxygen Species in Schizosaccharomyces pombe [PDF]

open access: yesPolish Journal of Microbiology, 2017
Schizosaccharomyces pombe cells of strains each carrying a deletion of one of the genes snf5, ypa1, pho7 and pas1 and of a strain overexpress­ing gene odr1, have been previously shown to grow in presence of the toxic glucose analogue 2-deoxyglucose ...
Akshay Vishwanatha   +2 more
doaj   +1 more source

Isolation and Characterization of Shewanella Phage Thanatos Infecting and Lysing Shewanella oneidensis and Promoting Nascent Biofilm Formation

open access: yesFrontiers in Microbiology, 2020
Species of the genus Shewanella are widespread in nature in various habitats, however, little is known about phages affecting Shewanella sp. Here, we report the isolation of phages from diverse freshwater environments that infect and lyse strains of ...
Maximilian Kreienbaum   +12 more
doaj   +1 more source

Rapid Extraction of Viral Nucleic Acids Using Rotating Blade Lysis and Magnetic Beads

open access: yesDiagnostics, 2022
The complex and lengthy protocol of current viral nucleic acid extraction processes limits their use outside laboratory settings. Here, we describe a rapid and reliable method for extracting nucleic acids from viral samples using a rotating blade and ...
Minju Bae   +7 more
doaj   +1 more source

The Holin-Endolysin Lysis System of the OP2-Like Phage X2 Infecting Xanthomonas oryzae pv. oryzae

open access: yesViruses, 2021
Most endolysins of dsDNA phages are exported by a holin-dependent mechanism, while in some cases endolysins are exported via a holin-independent mechanism.
Zhifeng Wu   +9 more
doaj   +1 more source

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