Integration of a virus membrane protein into the lipid bilayer of target cells as a prerequisite for immune cytolysis [PDF]
, 1979 Structural requirements for membrane antigens on target cells to mediate immune cytolysis were studied in a model system with purified membrane proteins from Semliki Forest virus (SFV). These SFV spike proteins were isolated in the form of detergent- and
Barz, D. +3 more
core +1 more source
Mycobacteriophage Lysis Enzymes: Targeting the Mycobacterial Cell Envelope
Viruses, 2018 Mycobacteriophages are viruses that specifically infect mycobacteria, which ultimately culminate in host cell death. Dedicated enzymes targeting the complex mycobacterial cell envelope arrangement have been identified in mycobacteriophage genomes, thus ...
Maria João Catalão, Madalena Pimentel
doaj +1 more source
VP3 Phage Combined with High Salt Promotes the Lysis of Biofilm-Associated Vibrio cholerae
Viruses, 2023 Cholera, caused by pathogenic Vibrio cholerae, poses a significant public health risk through water and food transmission. Biofilm-associated V. cholerae plays a crucial role in seasonal cholera outbreaks as both a reservoir in aquatic environments and a
Xu Li +4 more
doaj +1 more source
On the isolation of TI-plasmid from Agrobacterium tumefaciens [PDF]
, 1976 An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA ...
Dons, Johannes JM +7 more
core +2 more sources
Cytotoxicity of the Bacillus thuringiensis Crystal Protein against Mammalian Cells [PDF]
, 2002 The crystal proteins produced by Bacillus thuringiensis subsp, israelensis (Bti) and subsp. coreanensis A1519 strain were examined for the cytotoxicity against MOLT-4 and HeLa cells by MTT assay and LDH assay, The A1519 crystal proteins processed by ...
Akao, Tetsuyuki +5 more
core +1 more source
Evaluation of Lysis Methods for the Extraction of Bacterial DNA for Analysis of the Vaginal Microbiota [PDF]
, 2016 BACKGROUND:Recent studies on the vaginal microbiota have employed molecular techniques such as 16S rRNA gene sequencing to describe the bacterial community as a whole.
Blow, Frances +3 more
core +7 more sources
Nanoparticle Lysis of Cryptosporidium Oocysts
Methods and ProtocolsThe extraction of DNA from Cryptosporidium oocysts is challenging due to the robust oocyst wall. Nanoparticles have been applied to disinfect Cryptosporidium oocysts; here, we demonstrate the utilisation of nanoparticles to disrupt the oocyst wall to ...
Ameya Vaidya +3 more
doaj +1 more source
Characterization and Application of a Lytic Phage D10 against Multidrug-Resistant Salmonella
Viruses, 2021 Salmonella is a widely distributed foodborne pathogen that is a serious threat to human health. The accelerated development of drug resistance and the increased demand for natural foods invoke new biocontrol agents to limit contamination by multidrug ...
Zhiwei Li +7 more
doaj +1 more source
Shape-dependent optoelectronic cell lysis [PDF]
, 2014 We show an electrical method to break open living cells amongst a population of different cell types, where cell selection is based upon their shape.
Bao +28 more
core +1 more source
Crosstalk between the ribosome quality control‐associated E3 ubiquitin ligases LTN1 and RNF10
FEBS Letters, EarlyView.Loss of the E3 ligase LTN1, the ubiquitin‐like modifier UFM1, or the deubiquitinating enzyme UFSP2 disrupts endoplasmic reticulum–ribosome quality control (ER‐RQC), a pathway that removes stalled ribosomes and faulty proteins. This disruption may trigger a compensatory response to ER‐RQC defects, including increased expression of the E3 ligase RNF10 ...
Yuxi Huang +8 more
wiley +1 more source