Results 41 to 50 of about 7,486 (227)

Evolutionary Stabilization of Cooperative Toxin Production through a Bacterium-Plasmid-Phage Interplay

mBio, 2020
Colicins are toxins produced and released by Enterobacteriaceae to kill competitors in the gut. While group A colicins employ a division of labor strategy to liberate the toxin into the environment via colicin-specific lysis, group B colicin systems lack
Stefanie Spriewald, Eva Stadler, Burkhard A. Hense, Philipp C. Münch, Alice C. McHardy, Anna S. Weiss, Nancy Obeng, Johannes Müller, Bärbel Stecher   +8 more
doaj   +1 more source

Synthesis and Comparison of Linear Polymannosides for Direct Binding with Escherichia coli

Macromolecular Chemistry and Physics, Volume 224, Issue 24, December 2023., 2023
The multivalent mono and dimannosides based on linear polyglycerol identical in size and glycan density are synthesized. The label‐free microscale thermophoresis is employed to determine the binding affinities of multivalent scaffolds against whole bacteria. Both multivalent mannosides display remarkably very low nm binding constant (Kd) in contrast to
Natalie Hanheiser, Badri Parshad, Tatyana L. Povolotsky, Vinod Khatri, Katharina Achazi, Sumati Bhatia   +5 more
wiley   +1 more source

Two neglected but valuable genetic tools for Escherichia coli and other bacteria: In vivo cosmid packaging and inducible plasmid replication

Molecular Microbiology, Volume 120, Issue 6, Page 783-790, December 2023., 2023
Concatemers of a cosmid resulting from phage λ‐engendered rolling circle replication. Abstract In physiology and synthetic biology, it can be advantageous to introduce a gene into a naive bacterial host under conditions in which all cells receive the gene and remain fully functional.
John E. Cronan
wiley   +1 more source

Phage‐Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens

Advanced Science, Volume 10, Issue 24, August 25, 2023., 2023
In this report, a new PICI‐based platform is showcased to detect and identify bacteria. In contrast to other diagnostic approaches, this strategy can additionally distinguish whether the pathogens are alive. PICI‐based infection of target hosts is simple and efficient, allowing us to demonstrate the implementation of the technique in an easy‐to‐use ...
Rodrigo Ibarra‐Chávez, Julien Reboud, José R. Penadés, Jonathan M. Cooper   +3 more
wiley   +1 more source

Everything OLD is new again: How structural, functional, and bioinformatic advances have redefined a neglected nuclease family

Molecular Microbiology, Volume 120, Issue 2, Page 122-140, August 2023., 2023
OLD family nucleases are conserved ATP‐powered enzymes that function broadly in anti‐phage defense. In this review, we summarize our current understanding of OLD structure, function, and evolution, highlighting important advances gleaned from bioinformatics, genetics, and X‐ray crystallography.
Elena Wanvig Dot, Lynn C. Thomason, Joshua S. Chappie   +2 more
wiley   +1 more source

H-NS Mutation-Mediated CRISPR-Cas Activation Inhibits Phage Release and Toxin Production of Escherichia coli Stx2 Phage Lysogen

Frontiers in Microbiology, 2017
Shiga toxin-converting bacteriophages (Stx phages) carry the stx gene and convert nonpathogenic bacterial strains into Shiga toxin-producing bacteria. There is limited understanding of the effect that an Escherichia coli (E.
Q. Fu, Shiyu Li, Zhaofei Wang, Wenya Shan, Jingjiao Ma, Yuqiang Cheng, Hengan Wang, Yaxian Yan, Jianhe Sun   +8 more
semanticscholar   +1 more source

Effect of chromosomal integration on catalytic performance of a multi‐component P450 system in Escherichia coli

Biotechnology and Bioengineering, Volume 120, Issue 7, Page 1762-1772, July 2023., 2023
Chromosomal integration and expression of the genes encoding a bacterial P450 and two redox partners in recombinant E. coli strains resulted in up to 80% higher (S)‐ketamine conversion and more than 4‐fold increase in total turnover numbers compared to the respective plasmid‐based expression systems. Abstract Cytochromes P450 are useful biocatalysts in
U. Joost Luelf, Lisa M. Böhmer, Shengying Li, Vlada B. Urlacher   +3 more
wiley   +1 more source

Genomic Investigation of Lysogen Formation and Host Lysis Systems of the Salmonella Temperate Bacteriophage SPN9CC

Applied and Environmental Microbiology, 2013
To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized.
Hakdong Shin, Ju-Hoon Lee, Hyunjin Yoon, D. Kang, S. Ryu   +4 more
semanticscholar   +1 more source

Diversity, evolution and life strategies of CbK‐like phages

Environmental Microbiology, Volume 25, Issue 7, Page 1250-1264, July 2023., 2023
Abstract Caulobacter phage CbK has been extensively studied as a model system in virology and bacteriology. Lysogeny‐related genes have been found in each CbK‐like isolate, suggesting a life strategy of both lytic and lysogenic cycles. However, whether CbK‐related phages can enter lysogeny is still undetermined.
Ruijie Ma, Xiaowei Chen, Yingying Li, Nianzhi Jiao, Rui Zhang   +4 more
wiley   +1 more source

Large Preferred Region for Packaging of Bacterial DNA by phiC725A, a Novel Pseudomonas aeruginosa F116-Like Bacteriophage. [PDF]

PLoS ONE, 2017
Bacteriophage vB_PaeP_PAO1_phiC725A (short name phiC725A) was isolated following mitomycin C induction of C7-25, a clinical Pseudomonas aeruginosa strain carrying phiC725A as a prophage.
Christine Pourcel, Cédric Midoux, Yolande Hauck, Gilles Vergnaud, Libera Latino   +4 more
doaj   +1 more source