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Phage Endolysins as Promising and Effective Candidates for Use Against Uropathogenic <i>Escherichia coli</i>. [PDF]

open access: yesViruses
Wesołowski W   +8 more
europepmc   +1 more source
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Identification of Four Families of Peptidoglycan Lytic Transglycosylases

Journal of Molecular Evolution, 2001
The lytic transglycosylases are a class of autolysins which cleave the bacterial cell wall heteropolymer peptidoglycan (murein) to facilitate its biosynthesis and turnover. A search of the National Center for Biotechnology Information (NCBI) databases using the primary sequences of the six characterized lytic transglycosylases of Escherichia coli, a ...
Anthony J. Clarke, Neil T. Blackburn
openaire   +3 more sources

Assay for Lytic Transglycosylases: A Family of Peptidoglycan Lyases

Analytical Biochemistry, 2000
An assay has been developed to monitor the activity of the lytic transglycosylases which does not involve the use of radiolabel. Samples of lytic transglycosylase were incubated with isolated and purified insoluble peptidoglycan as substrate for varying lengths of time.
Anthony J. Clarke, Neil T. Blackburn
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‘Holy’ proteins II: the soluble lytic transglycosylase

Current Opinion in Structural Biology, 1994
Enzymes involved in the metabolism of the bacterial cell wall peptidoglycan are excellent targets for antibiotics. Penicillins and related beta-lactam antibiotics inhibit the enzymes that act on the peptide cross-links of the peptidoglycan. The X-ray structure of the transglycosylase revealed a two-layered ring of alpha-helices in a right-handed ...
Bauke W. Dijkstra   +1 more
openaire   +3 more sources

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