Results 201 to 210 of about 77,614 (254)

Isolation of F-specific <i>Inovirus</i> filamentous phages from environmental sewage samples. [PDF]

MethodsX
Theriault N, Cook BWM, Theriault SS, Court DA.   +3 more
europepmc   +1 more source

A Photoresponsive Hybrid of Viruses and Supramolecular Peptide Fibers for Multidimensional Control of Patterning and Infection. [PDF]

Angew Chem Int Ed Engl
Yaguchi A, Uchida N, Miura D, Watanabe G, Hiramatsu H, Ajioka I, Matsubara T, Sato T, Enkhzaya C, Itani S, Saito T, Muraoka T.   +11 more
europepmc   +1 more source

Plating Bacteriophage M13

Cold Spring Harbor Protocols, 2017
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Michael R, Green, Joseph, Sambrook
openaire   +3 more sources

Structural characterization of membrane insertion of M13 procoat, M13 coat, and Pf3 coat proteins [PDF]

Biochemistry, 1993
A new, simple, and efficient purification method has been developed for the extremely hydrophobic M13 procoat, M13 coat, and Pf3 coat proteins. Homogeneous preparations were obtained in 2-propanol/0.1% TFA, where M13 coat protein is found to be dissolved in a monomeric form, and the two other proteins as dimers or trimers.
E, Thiaudière, M, Soekarjo, E, Kuchinka, A, Kuhn, H, Vogel   +4 more
openaire   +3 more sources

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Plating Bacteriophage M13

Cold Spring Harbor Protocols, 2006
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Joseph, Sambrook, David W, Russell
openaire   +2 more sources

Replication of bacteriophage M13

Journal of Molecular Biology, 1977
The origin for bacteriophage M13 single-strand synthesis has been localized by two different experimental approaches: (1) localization of the discontinuity in late life-cycle RFII ‡ , one of the initial product molecules during single-strand synthesis, and (2) determination of a gradient of radioactive label in RFI pulselabeled during single-strand ...
Sidney V. Suggs, Dan S. Ray
openaire   +2 more sources

M13-102: A vector for facilitating construction and improving quality of M13 shotgun libraries

Gene, 1996
A modified vector, M13-102, is described which utilizes the previously reported M13-100 direct selection strategy for shotgun cloning [Guilfoyle and Smith, Nucleic Acids Res. 22 (1994) 100-107]. In these vectors, direct selection replaces the need for phosphatase treatment of vector DNA and is achieved by insertional inactivation of M13 gene X.
D, Chen, A F, Johnson, J M, Severin, D R, Rank, L M, Smith, R A, Guilfoyle   +5 more
openaire   +2 more sources

M13 Bacteriophages as Bioreceptors in Biosensor Device

2019
New recognition probes sensible, specific and robust is one of the major problems of biosensor assay. Detection biosensors has utilized antibodies or enzymes as bioreceptors; however, these have numerous disadvantages of limited binding sites and physico-chemical instabilities, can negatively affect capture and detection of target in diagnostic device.
De Plano, Laura M., Franco, Domenico, Rizzo, Maria Giovanna, Crea, Sara, Messina, Grazia M. L., Marletta, Giovanni, Guglielmino, Salvatore P. P.   +6 more
openaire   +3 more sources

Engineering M13 for phage display

Biomolecular Engineering, 2001
Phage display is achieved by fusing polypeptide libraries to phage coat proteins. The resulting phage particles display the polypeptides on their surfaces and they also contain the encoding DNA. Library members with particular functions can be isolated with simple selections and polypeptide sequences can be decoded from the encapsulated DNA.
openaire   +2 more sources