Results 211 to 220 of about 83,983 (257)
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1986
This chapter focuses on a method of sequencing M13 clones that allows the determination of the nucleotide sequence of inserts cloned into M13 vectors. A 17-mer oligonucleotide primer is annealed to the M13 vector in a position adjacent to the polylinker cloning region.
Leonard G. Davis +2 more
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This chapter focuses on a method of sequencing M13 clones that allows the determination of the nucleotide sequence of inserts cloned into M13 vectors. A 17-mer oligonucleotide primer is annealed to the M13 vector in a position adjacent to the polylinker cloning region.
Leonard G. Davis +2 more
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Engineering M13 for phage display
Biomolecular Engineering, 2001Phage display is achieved by fusing polypeptide libraries to phage coat proteins. The resulting phage particles display the polypeptides on their surfaces and they also contain the encoding DNA. Library members with particular functions can be isolated with simple selections and polypeptide sequences can be decoded from the encapsulated DNA.
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M13 Bacteriophages as Bioreceptors in Biosensor Device
2019New recognition probes sensible, specific and robust is one of the major problems of biosensor assay. Detection biosensors has utilized antibodies or enzymes as bioreceptors; however, these have numerous disadvantages of limited binding sites and physico-chemical instabilities, can negatively affect capture and detection of target in diagnostic device.
De Plano, Laura M. +6 more
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Replication of the Small Coliphage M13: Evidence for Long-living M13 Specific Messenger RNA
Nature, 1970THE stability of messenger RNA has been the object of intense investigations. In the Escherichia coli system, predominantly the mRNAs for inducible enzymes or mRNAs specified by lytic or inducible phages have been studied. All such mRNAs were found to have half lives of the order of several minutes, even when measured on the basis of physical rather ...
R, Jaenisch, E, Jacob, P H, Hofschneider
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Structural characterization of membrane insertion of M13 procoat, M13 coat, and Pf3 coat proteins
Biochemistry, 1993A new, simple, and efficient purification method has been developed for the extremely hydrophobic M13 procoat, M13 coat, and Pf3 coat proteins. Homogeneous preparations were obtained in 2-propanol/0.1% TFA, where M13 coat protein is found to be dissolved in a monomeric form, and the two other proteins as dimers or trimers.
E, Thiaudière +4 more
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Growing Bacteriophage M13 in Liquid Culture
Cold Spring Harbor Protocols, 2017Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Michael R, Green, Joseph, Sambrook
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Growing Bacteriophage M13 in Liquid Culture
Cold Spring Harbor Protocols, 2006Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Joseph, Sambrook, David W, Russell
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????????-?????????? ?????????????????????? ???????????????????? ???????? M13
2019???????????????? ????????-?????????? ?????????????????????? ???????? M13mp8 ?? mp9. ?????????????? ???????????????????????????????????? ?????????? ?????????????????????? ???? 14 ???? 34 % ?????????????? ???????????????? ????????????. ?????? ????????-???????? ?????????????????? ?????????????? ?????? ~640 ??. ??., ???????????????????? ????????????????????
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Cloning into Bacteriophage M13 Vectors
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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