Intermittent Surface Oxygenation Results in Similar Mitochondrial Protection and Maintenance of Aerobic Metabolism as Compared to Continuous Oxygenation during Hypothermic Machine Kidney Machine Perfusion. [PDF]
Darius T +14 more
europepmc +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Research on machine to machine (M2M) load control
In recent years,Machine Type Communication(MTC)can also be referred to as Machine to Machine(M2M),which is a new kind of communication service,receives widespread attention gradually and is a hot topic in the 3 GPP standardization conference.However,due ...
ZHANG Jun, DONG Jianping
doaj +1 more source
Metabolic detection of malignant brain gliomas through plasma lipidomic analysis and support vector machine-based machine learning. [PDF]
Zhou J +10 more
europepmc +1 more source
Digital twins to accelerate target identification and drug development for immune‐mediated disorders
Digital twins integrate patient‐derived molecular and clinical data into personalised computational models that simulate disease mechanisms. They enable rapid identification and validation of therapeutic targets, prediction of drug responses, and prioritisation of candidate interventions.
Anna Niarakis, Philippe Moingeon
wiley +1 more source
Timed Verification of Machine-to-Machine communications
AbstractMachine-to-Machine communications (M2M) is emerging as a new technology for next generation of communicating systems. In such M2M based systems, a large number of intelligent machines share information and make collaborative decisions without human interventions.
Gharbi, Ghada +2 more
openaire +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
A NOMA-Enhanced Reconfigurable Access Scheme With Device Pairing for M2M Networks
This paper aims to address the distinct requirements of machine-to-machine networks, particularly heterogeneity and massive transmissions. To this end, a reconfigurable medium access control with the ability to choose a proper access scheme with the ...
Atoosa Dalili Shoaei +2 more
doaj +1 more source
Identifying Hybrid DDoS Attacks in Deterministic Machine-to-Machine Networks on a Per-Deterministic-Flow Basis. [PDF]
Chen YH, Lai YC, Zhou KZ.
europepmc +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source

