Results 211 to 220 of about 5,046 (240)
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DNA Microinjection into the Macronucleus of Paramecium
Cold Spring Harbor Protocols, 2010INTRODUCTIONThis protocol describes a highly efficient procedure for transforming the vegetative macronucleus of Paramecium tetraurelia by DNA microinjection. Any microinjected DNA will be replicated without the need for specific origins and can be stably maintained at a wide range of copy numbers throughout vegetative growth as minichromosomes that ...
Janine, Beisson +10 more
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Chromatin Elimination and the Ciliate Macronucleus
The American Naturalist, 1947Chromatin elimination is known to occur in a number of ani- mals and is apparently of different kinds. First, there is the loss of parts of chromosomes in Asccris megalocephala where, in the early cleavages of the egg, the blastomeres destined to give rise to the somatic tissues lose the ends of their chromosomes and these ends are absorbed by the ...
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Double paths of macronucleus differentiation at conjugation in Blepharisma japonicum
European Journal of Protistology, 1991Nuclear phenomena at conjugation of the multimicronucleate ciliate Blepharisma japonicum were investigated by using fluorescence microscopy. Several hours after cells united in pairs, micronuclei differentiated into meiotic micronuclei and "somatomicronuclei".
A, Miyake, V, Rivola, T, Harumoto
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Reorganization of microtubules in the amitotically dividing macronucleus ofTetrahymena
Cell Motility and the Cytoskeleton, 2000We developed a modified immunofluorescence protocol that permitted visualization of microtubules inside the macronucleus of the ciliate Tetrahymena. Although the amitotically dividing macronucleus lacks a spindle, an elaborate system of microtubules is assembled inside the macronucleus and between the macronucleus and the cortex. Microtubules could not
K, Fujiu, O, Numata
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Draft macronucleus genome of Euplotes crassus ciliate
Molecular Biology, 2012Basic bioinformatics analysis has been performed on the draft macronucleus genome and transcriptome of the Euplotes crassus ciliate. More than a quarter of E. crassus genes contain several exons. Among introns, a large fraction of tiny introns was revealed with the length of 20–30 nucleotides. We determined 63 cases of alternative splicing and found 14
D. V. Vinogradov +6 more
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Location of rDNA in the heteromeric macronucleus of Chilodonella steini
European Journal of Protistology, 1990As it was shown previously the chromatin of the heteromeric macronucleus of Chilodonella steini consists of two specific zones, the outer one (the orthomere) made up from late replicating electron dense material, and the inner zone (the paramere) of medium electron density and early replicating, with a concentration of electron dense material called ...
S, Radzikowski, G, Steinbrück
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Le Macronucleus Hétéromere de Quelques Ciliés
The Journal of Protozoology, 1957SUMMARY. In ciliates belonging to the two gymnostome families Chlamydodontidae and Dysteriidae as well as to the order Chonotrichida the macronucleus is composed of two parts which are distinct but in very close juxtaposition. One part, here called “orthomerous,” contains granules or microsomes of desoxyribonucleoprotein and several nucleoli; it thus ...
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Localization of acetylated histone H4 in the macronucleus of Euplotes
Chromosoma, 1991Antisera specific to acetylated and unacetylated N-terminal domains of histone H4 were employed to map the spatial distribution of exposed antigenic determinants in the macronucleus of Euplotes eurystomus. Sites of putative transcription-related acetylation appear to reside in the regions between condensed chromatin granules and disappear during ...
Olins, D. +5 more
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Induced elimination of DNA from macronucleus of Tetrahymena pyriformis
Experimental Cell Research, 1972Abstract The effect of thymidine starvation on replicating DNA in Tetrahymena pyriformis is described. Cells were pulse-labelled with 3 H-thymidine just prior to inhibition of replication. Autoradiographic analysis has shown that the fraction of labelled DNA—which was in replication at the time of inhibition—is eliminated from the macronucleus ...
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The Ciliary Protein
AbstractThe intraflagellar transportIFT57 protein is essential for ciliary growth and maintenance. Also known asHIPPI, humanIFT57 can be translocated to the nucleus via a molecular partner of the Huntingtin, Hip1, inducing gene expression changes.
Shi, Lei +3 more
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