Results 111 to 120 of about 30,016 (261)

A force calibration standard for magnetic tweezers

Review of Scientific Instruments, 2014
To study the behavior of biological macromolecules and enzymatic reactions under force, advances in single-molecule force spectroscopy have proven instrumental. Magnetic tweezers form one of the most powerful of these techniques, due to their overall simplicity, non-invasive character, potential for high throughput measurements, and large force range ...
Zhongbo Yu, David Dulin, Jelmer Cnossen, Mariana Köber, Maarten M. van Oene, Orkide Ordu, Bojk A. Berghuis, Toivo Hensgens, Jan Lipfert, Nynke H. Dekker   +9 more
openaire   +5 more sources

A Linearly Deformable Pneumatic Scalable Microgripper for Universal Mid‐Air Micromanipulation

Advanced Science, EarlyView.
This work presents a fully 3D printed soft microgripper capable of active adhesion control through a pneumatic system. Its modular design can reach a high adhesion switching ratio of 373 and free release of micro‐objects at 100 μm$\umu \rm {m}$ dimension.
Jiawei Yi, Wissem Haouas, Gwenn Ulliac, Kanty Rabenorosoa   +3 more
wiley   +1 more source

Material‐Induced Nuclear Deformation Controls Chromatin Architecture in Adipose Stem Cells

Advanced Science, EarlyView.
Tuning cell and cytoskeleton mechanics modulated nuclear shape and heterochromatin organization in ASCs. Distinct cytoskeletal architectures induced nuclear morphologies from oblate to prolate ellipsoids. Large elongated cells with a structured actin cap exhibited high nuclear strain, driving nuclear envelope deformation and heterochromatin ...
Carlo F. Natale, Luca Messina, Valeria Panzetta, Stefania Saporito, Costantino Menna, Maurizio Ventre, Paolo A. Netti   +6 more
wiley   +1 more source

Synthesis and Characterization of Unusual S=1 Fe(0)‐Silyl Complexes Supported by Styrene Ligands

Angewandte Chemie, EarlyView.
Despite the long‐standing importance of iron‐silicon bonds as related to catalysis, high‐spin Fe(0)‐silyl complexes remain a historical synthetic challenge in the field. Herein, we report the first series of high‐spin S = 1, styrene‐stabilized Fe(0)‐silyl complexes followed by extensive characterization via Mössbauer spectroscopy, single crystal X‐ray ...
Alexis K. Bauer, Agamemnon E. Crumpton, Michael L. Neidig   +2 more
wiley   +2 more sources

Advanced Human Immune Cell‐Organoid Co‐Cultures for Functional Testing of Cancer Nanovaccines

Advanced Science, EarlyView.
Pancreatic ductal adenocarcinoma remains difficult to treat. We establish an organoid–immune co‐culture using patient‐derived organoids and matched T‐cells to assess cancer vaccines. A mesothelin‐targeted nanovaccine activates antigen‐specific T‐cells, increases IFN‐γ, and targets MSLN+ organoids.
Nathalia Ferreira, David Agorku, Andre Rosa, Julia Roosz, Lena Christ, Nicole Anderle, Ajinkya Kulkarni, Abir Hussein, Sana S. Sayedipour, Omar F. Luna, Tobias Legler, Philipp Ströbel, Fernando Albericio, Luis Cruz, Phillipp Beckhove, Peter Loskill, Frauke Alves, M. Andrea Markus, Fernanda Ramos‐Gomes   +18 more
wiley   +1 more source

Homology sensing via non-linear amplification of sequence-dependent pausing by RecQ helicase

eLife, 2019
RecQ helicases promote genomic stability through their unique ability to suppress illegitimate recombination and resolve recombination intermediates. These DNA structure-specific activities of RecQ helicases are mediated by the helicase-and-RNAseD like C-
Yeonee Seol, Gábor M Harami, Mihály Kovács, Keir C Neuman   +3 more
doaj   +1 more source

AIEgen‐Based Proactive Early Warning System and Precise Treatment Strategy for Monkeypox Prevention and Control

Advanced Science, EarlyView.
An intelligent early warning and treatment system for monkeypox, utilizing a sprayable AIEgen‐ liquid dressing (AIE@LD) that responds to lesion pH. Integrated with a smartphone app and cloud platform, it enables real‐time infection detection via fluorescence imaging and triggers on‐demand photodynamic therapy under white light, effectively eradicating ...
Wei Wang, Zining Liu, Mengjun Li, Judun Zheng, Ruilin Zhang, Ke Liu, Xiaoxue Li, Shaoying Wen, Jingze Yu, Meijia Pan, Fengbo Ma, Kun Zhou, Zheng Zhao, Chenguang Shen, Yuhui Liao   +14 more
wiley   +1 more source

Discovery of an Adaptive Neuroimmune Response Driving Itch and Fast Tick Removal with Implications for Preventing Pathogen Transmission

Advanced Science, EarlyView.
Doehl et al. discovered an adaptive neuroimmune mechanism that induces itch in tick‐exposed guinea pigs, enabling rapid tick removal. This itch‐induced tick removal (IITR) is mediated by an adaptive cellular immune response and is independent of IgG, IgE, or TRPV1.
Johannes S. P. Doehl, Tiago D. Serafim, Serena Doh, Charles S. Grugan, Eva Iniguez, Luana Rogerio, Ronja Frigard, Ranadhir Dey, Pedro Cecilio, Xinglong Gu, Pang‐Yen Tseng, Aline da Silva Moreira, Mahnaz Minai, James Oristian, Hans Ackerman, Steven Brooks, Caroline Percopo, Siu‐Ping Ng, Derron A. Alves, Lucas Tirloni, Jennifer M. Anderson, Adriana Marques, Fabiano Oliveira, Shaden Kamhawi, Daniel E. Sonenshine, José M. C. Ribeiro, Mark Hoon, Jesus G. Valenzuela   +27 more
wiley   +1 more source

Flexible Thin‐Film Heater Composed of Laser‐Induced Graphene and Poly(D, L‐Lactic Acid) with Electro‐ and Photo‐Thermal Properties toward Cancer Thermotherapy

Advanced Electronic Materials, EarlyView.
This study introduces a thin‐film‐type heat‐generating device that uses laser induced graphene (LIG) on a poly(D, L‐lactic) acid film, which realizes both resistance heating by direct current application and photothermal conversion heating by near‐infrared light irradiation.
Masato Saito, Sota Harada, Ran Imai, Kon Son, Toshinori Fujie   +4 more
wiley   +1 more source

The mechanism of variability in transcription start site selection

eLife, 2017
During transcription initiation, RNA polymerase (RNAP) binds to promoter DNA, unwinds promoter DNA to form an RNAP-promoter open complex (RPo) containing a single-stranded ‘transcription bubble,’ and selects a transcription start site (TSS).
Libing Yu, Jared T Winkelman, Chirangini Pukhrambam, Terence R Strick, Bryce E Nickels, Richard H Ebright   +5 more
doaj   +1 more source