Results 231 to 240 of about 10,645 (268)
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Exploring Condensins with Magnetic Tweezers
2017The signature activity of condensins as DNA reshaping machines is their ability to impose the giant loop architecture onto the chromosome. At the heart of this activity lies the propensity of the proteins to assemble into macromolecular clusters that bring distant DNA segments together.
Rupa, Sarkar, Valentin V, Rybenkov
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Magnetic Tweezers in Cell Biology
2007We discuss herein the theory as well as some design considerations of magnetic tweezers. This method of generating force on magnetic particles bound to biological entities is shown to have a number of advantages over other techniques: forces are exerted in noncontact mode, they can be large in magnitude (order of 10 nanonewtons), and adjustable in ...
Monica, Tanase +2 more
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Particles sorting in micro-channel using magnetic tweezers and optical tweezers
The 9th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS), 2014This study evaluates a method for separating magnetic microparticles in a micro channel by using embedded inverted-laser tweezers, a microflow pump, and a micro magnet. Various particles were separated using optical and/or magnetic tweezers, and were identified and counted to determine the dependence of the sorting rate on the channel flow velocity ...
Yung-Chiang Chung +3 more
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Probing Chromatin Structure with Magnetic Tweezers
2018Magnetic tweezers form a unique tool to study the topology and mechanical properties of chromatin fibers. Chromatin is a complex of DNA and proteins that folds the DNA in such a way that meter-long stretches of DNA fit into the micron-sized cell nucleus.
Artur, Kaczmarczyk +4 more
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Magnetic Tweezers for Single-Molecule Manipulation
2011Magnetic tweezers provide a versatile tool enabling the application of force and torque on individual biomolecules. Magnetic tweezers are uniquely suited to the study of DNA topology and protein-DNA interactions that modify DNA topology. Perhaps due to its presumed simplicity, magnetic tweezers instrumentation has been described in less detail than ...
Yeonee, Seol, Keir C, Neuman
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Biofunctional Magnetic Tweezers
Science, 2001MATERIALS SCIENCE The viscoelastic properties of cells and intracellular transport forces have been studied with micrometer-sized transducers that can handle relatively large forces (tens of nanonewtons). Abdelghani-Jacquin et al. have made magnetic cobalt and iron particles more cell-friendly by plating them with gold in a spontaneous electrodeless ...
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Magnetic tweezers principles and promises
Magnetic tweezers have become popular with the outbreak of single molecule micromanipulation: catching a single molecule of DNA, RNA or a single protein and applying mechanical constrains using micron-size magnetic beads and magnets turn out to be easy. Various factors have made this possible: the fact that manufacturers have been preparing these beadsVincent, Croquette +3 more
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Magnetically actuated micromechanical tweezers
Sensors and Actuators A: Physical, 2011Abstract Two planar actuators with magnetic thin films are used for magnetic tweezers. The planar actuators consisting of a pair of a 75 × 0.8 × 0.3 μm 3 silicon oxide beam and a 72 × 13 × 0.3 μm 3 silicon oxide plate deposited with a 65 × 4 × 0.1 μm 3 Ni magnetic thin film are successfully fabricated and successfully gripped to a single NPC-tw01 ...
Yi-Ping Hsieh +3 more
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Applications of Magnetic Tweezers to Studies of NAPs
2017Nucleoid-associated proteins (NAPs) are important factors in shaping bacterial nucleoid and regulating global gene expression. A great deal of insights into NAPs can be obtained through studies using single DNA molecule, which has been made possible owing to recent rapid development of single-DNA manipulation techniques.
Ricksen S, Winardhi, Jie, Yan
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Magnetic tweezers for DNA micromanipulation
Review of Scientific Instruments, 2000We detail the design of an electromagnetic assembly capable of generating a constant magnetic field superimposed to a large magnetic field gradient (between 40 and 100 T/m), which was uniform over a large gap (between 1.5 and 2 cm). Large gaps allowed the use of wide high numerical-aperture lenses to track microspheres attached to DNA molecules with an
Charbel Haber, Denis Wirtz
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