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Agrobacterium-Mediated Transformation of Maize (Zea mays) Immature Embryos
2013Agrobacterium tumefaciens-mediated transformation is one of the most efficient and simple gene delivery systems for genetic improvement and biology studies in maize. This system has become more widely used by both public and private laboratories. However, transformation efficiencies vary greatly from laboratory to laboratory for the same genotype. Here,
Hyeyoung, Lee, Zhanyuan J, Zhang
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Sampling the Maize (Zea mays) Leaf Microbiome
Cold Spring Harbor ProtocolsThe microbiota of maize leaves can be beneficial or detrimental to the host. Foliar diseases are the most obvious detrimental impact of the leaf microbiome, though more subtle effects of the normal (nondisease) community are an active area of research. This protocol describes two specific methodologies to sample the maize leaf microbiome: one sampling ...
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Anther culture of maize (Zea mays L.)
2003The potential of doubled haploids (DH) in maize breeding has long been recognised. By the use of DH-producing techniques various gene combinations can be fixed in homozygous form in a short time. Moreover, DH lines possessing high fertility and favourable agronomic characters can be utilized directly in heterosis breeding to produce new hybrids which ...
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