Competitive interactions of ligands and macromolecular crowders with maltose binding protein. [PDF]
PLoS ONE, 2013 Cellular signaling involves a cascade of recognition events occurring in a complex environment with high concentrations of proteins, polysaccharides, and other macromolecules.
Andrew C Miklos +2 more
doaj +7 more sources
Folding of maltose binding protein outside of and in GroEL. [PDF]
Proc Natl Acad Sci U S A, 2018 Significance The GroEL/ES chaperonin is known to prevent protein aggregation during folding by passive containment within the central cavity. The possible role of more active intervention is controversial.
Ye X, Mayne L, Kan ZY, Englander SW.
europepmc +6 more sources
Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems. [PDF]
PLoS ONE, 2016 Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many proteins can be expressed easily, some proteins, sub-domains, and mutant protein versions ...
Raphael Reuten +7 more
doaj +3 more sources
A Maltose-Binding Protein Fusion Construct Yields a Robust Crystallography Platform for MCL1. [PDF]
PLoS ONE, 2015 Crystallization of a maltose-binding protein MCL1 fusion has yielded a robust crystallography platform that generated the first apo MCL1 crystal structure, as well as five ligand-bound structures.
Matthew C Clifton +17 more
doaj +3 more sources
In Silico Generation of Peptides by Replica Exchange Monte Carlo: Docking-Based Optimization of Maltose-Binding-Protein Ligands. [PDF]
PLoS ONE, 2015 Short peptides can be designed in silico and synthesized through automated techniques, making them advantageous and versatile protein binders. A number of docking-based algorithms allow for a computational screening of peptides as binders.
Anna Russo +8 more
doaj +3 more sources
CpG ODN1826 as a Promising Mucin1-Maltose-Binding Protein Vaccine Adjuvant Induced DC Maturation and Enhanced Antitumor Immunity. [PDF]
Int J Mol Sci, 2018 Mucin 1 (MUC1), being an oncogene, is an attractive target in tumor immunotherapy. Maltose binding protein (MBP) is a potent built-in adjuvant to enhance protein immunogenicity.
Jie J +7 more
europepmc +4 more sources
Prokaryotic soluble expression and purification of bioactive human fibroblast growth factor 21 using maltose-binding protein. [PDF]
Sci Rep, 2017 Human fibroblast growth factor 21 (hFGF21) has been characterized as an important regulator of glucose and lipid metabolism homeostasis. Here, to produce hFGF21 efficiently in Escherichia coli, the expression and solubility of hFGF21 were tested and ...
Nguyen AN +13 more
europepmc +4 more sources
Structure of an engineered β-lactamase maltose binding protein fusion protein: insights into heterotropic allosteric regulation. [PDF]
PLoS ONE, 2012 Engineering novel allostery into existing proteins is a challenging endeavor to obtain novel sensors, therapeutic proteins, or modulate metabolic and cellular processes.
Wei Ke +8 more
doaj +4 more sources
Accessing a hidden conformation of the maltose binding protein using accelerated molecular dynamics.
PLoS Computational Biology, 2011 Periplasmic binding proteins (PBPs) are a large family of molecular transporters that play a key role in nutrient uptake and chemotaxis in Gram-negative bacteria.
Denis Bucher +3 more
doaj +3 more sources
Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins.
J Struct Biol, 2016 We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations that enhance MBP solubility and affinity purification ...
Bokhove M +9 more
europepmc +6 more sources