Results 131 to 140 of about 942,075 (278)

Engineering of Cyclodextrin Product Specificity and pH Optima of the Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1 [PDF]

, 1998
The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis.
Bauke W. Dijkstra, Bhat, Bradford, Brünger, Davies, Engh, Fujiwara, Haeckel, Harata, Jones, Joost C.M. Uitdehaag, Kabsch, Klein, Klein, Kleywegt, Knegtel, Knegtel, Koshland, Lamzin, Laskowski, Lawson, Lazzaroni, Lubbert Dijkhuizen, Monma, Nakamura, Nakamura, Norman, Pedersen, Penninga, Qian, Read, Reinetta M. Buitelaar, Richèle D. Wind, Sambrook, Sanger, Schmid, Starnes, Strokopytov, Strokopytov, Svensson, Takase, Takusagawa, Tronrud, Wind, Yanisch-Perron   +44 more
core   +2 more sources

Expression of Human ACE2 N-terminal Domain, Part of the Receptor for SARS-CoV-2, in Fusion With Maltose-Binding Protein, E. coli Ribonuclease I and Human RNase A. [PDF]

Front Microbiol, 2021
Xu SY, Fomenkov A, Chen TH, Yigit E.
europepmc   +2 more sources

Solute channels of the outer membrane: from bacteria to chloroplasts [PDF]

, 2007
Chloroplasts, unique organelles of plants, originated from endosymbiosis of an ancestor of today's cyanobacteria with a mitochondria-containing host cell. It is assumed that the outer envelope membrane, which delimits the chloroplast from the surrounding
Aljamal J.A., Benz R., Blumenthal A., Drea S.C., Fischer K., Murcha M.W., Pilsl H., Umeda H., Zizi M.   +8 more
core   +1 more source

Engineering of Bacillus thuringiensis Cry Proteins to Enhance the Activity against Western Corn Rootworm

Toxins, 2019
A novel Bacillus thuringiensis Cry protein, Cry8Hb, active against Diabrotica virgifera virgifera (Western corn rootworm, WCRW) was discovered. Unexpectedly, the anti-rootworm activity of the Cry8Hb toxin was enhanced significantly by fusing Escherichia ...
Jingtong Hou, Ruth Cong, Michi Izumi-Willcoxon, Hana Ali, Yi Zheng, Ericka Bermudez, Mark McDonald, Mark Nelson, Takashi Yamamoto   +8 more
doaj   +1 more source

Alterations and mechanistic insights of gut microbiota and its metabolites in type 2 diabetes mellitus and Alzheimer's disease

iMetaOmics, EarlyView.
This narrative review uniquely addresses how gut microbiota‐derived metabolites mediate overlapping pathologies of insulin resistance, neuroinflammation, and amyloidogenesis in type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD), proposing a framework for dual therapeutic targeting.
Guangyi Xu, Yu An, Yage Du, Zhaoming Cao, Jie Zheng, Jingya Wang, Tingyi Li, Xingen Lei, Yanhui Lu   +8 more
wiley   +1 more source

Giardia Cyst Wall Protein 1 Is a Lectin That Binds to Curled Fibrils of the GalNAc Homopolymer [PDF]

, 2010
The infectious and diagnostic stage of Giardia lamblia (also known as G. intestinalis or G. duodenalis) is the cyst. The Giardia cyst wall contains fibrils of a unique β-1,3-linked N-acetylgalactosamine (GalNAc) homopolymer and at least three cyst wall ...
Bullitt, Esther, Carpentieri, Andrea, Chatterjee, Aparajita, Costello, Catherine E., Ratner, Daniel M., Robbins, Phillips W., Samuelson, John   +6 more
core   +4 more sources

Programming xenon diffusion in maltose-binding protein. [PDF]

Biophys J, 2022
Zhao Z, Rudman NA, He J, Dmochowski IJ.
europepmc   +1 more source

Photoaffinity labeling coupled to MS to identify peptide biological partners: Secondary reactions, for better or for worse?

Mass Spectrometry Reviews, EarlyView.
Abstract Affinity photolabeling is a smart method to study noncovalent and transient interactions and provide a submolecular picture of the contacts between interacting partners. In this review, we will focus on the identification of peptide partners using photoaffinity labeling coupled to mass spectrometry in different contexts such as in vitro with a
Astrid Walrant, Emmanuelle Sachon
wiley   +1 more source

ATPase Regulation in the Maltose Transporter [PDF]

, 2011
This thesis investigates the mechanism of activity-coupling in the maltose transporter of Escherichia coli (MalFGK2); the way ATP hydrolysis is prevented in the absence of maltose, and then enabled to drive maltose transport.
Gould, Alister D
core   +1 more source

Structural and thermodynamic approach to peptide immunogenicity [PDF]

, 2008
In the conventional paradigm of humoral immunity, B cells recognize their cognate antigen target in its native form. However, it is well known that relatively unstable peptides bearing only partial structural resemblance to the native protein can trigger
Camacho, CJ, Katsumata, Y, Ascherman, DP
core   +1 more source