Results 141 to 150 of about 940,374 (326)

Recombinant protein immobilisation and display by alginate : a thesis presented in partial fulfilment of the requirements of the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand. [PDF]

, 2016
Biopolymers are a diverse group of organic materials with important applications in a number of industries. Their ability to adsorb and encapsulate compounds has been widely utilised in both biotechnologies and pharmaceuticals.
Jameson, Andrew
core  

Recent Advances in Enzymes and Chemoenzymatic Synthesis of Tetrasaccharide Linkage Region of Proteoglycans

ChemBioChem, EarlyView.
The tetrasaccharide linkage region of proteoglycans is synthesized through a series of well‐controlled enzymatic reactions in nature. The expression, activity, and substrate profile of these biosynthetic enzymes and the latest development of chemoenzymatic synthesis of linkage region‐bearing glycopeptides are summarized in this review.
Po‐han Lin, Xuefei Huang
wiley   +1 more source

Expression of Human ACE2 N-terminal Domain, Part of the Receptor for SARS-CoV-2, in Fusion With Maltose-Binding Protein, E. coli Ribonuclease I and Human RNase A. [PDF]

Front Microbiol, 2021
Xu SY, Fomenkov A, Chen TH, Yigit E.
europepmc   +2 more sources

Engineering and Structural Elucidation of a Sac7d‐Derived IgG Fc‐Specific Affitin and Its Application for the Light‐Controlled Affinity Purification of Antibodies

ChemBioChem, EarlyView.
An affitin derived from the Sac7d scaffold enables the light‐controlled affinity purification of antibodies under physiological conditions, thus overcoming limitations of protein A chromatography. X‐ray crystallographic analysis and affinity measurements reveal complex formation with Fc at the CH2/CH3 junction, similar to protein A/G and FnRn. Combined
Felix Veitl, Andreas Eichinger, Peter Mayrhofer, Markus R. Anneser, Mauricio Testanera, Kilian Rauscher, Matthias Lenz, Arne Skerra   +7 more
wiley   +1 more source

Engineering of Bacillus thuringiensis Cry Proteins to Enhance the Activity against Western Corn Rootworm

Toxins, 2019
A novel Bacillus thuringiensis Cry protein, Cry8Hb, active against Diabrotica virgifera virgifera (Western corn rootworm, WCRW) was discovered. Unexpectedly, the anti-rootworm activity of the Cry8Hb toxin was enhanced significantly by fusing Escherichia ...
Jingtong Hou, Ruth Cong, Michi Izumi-Willcoxon, Hana Ali, Yi Zheng, Ericka Bermudez, Mark McDonald, Mark Nelson, Takashi Yamamoto   +8 more
doaj   +1 more source

Engineering of Cyclodextrin Product Specificity and pH Optima of the Thermostable Cyclodextrin Glycosyltransferase from Thermoanaerobacterium thermosulfurigenes EM1 [PDF]

, 1998
The product specificity and pH optimum of the thermostable cyclodextrin glycosyltransferase (CGTase) from Thermoanaerobacterium thermosulfurigenes EM1 was engineered using a combination of x-ray crystallography and site-directed mutagenesis.
Bauke W. Dijkstra, Bhat, Bradford, Brünger, Davies, Engh, Fujiwara, Haeckel, Harata, Jones, Joost C.M. Uitdehaag, Kabsch, Klein, Klein, Kleywegt, Knegtel, Knegtel, Koshland, Lamzin, Laskowski, Lawson, Lazzaroni, Lubbert Dijkhuizen, Monma, Nakamura, Nakamura, Norman, Pedersen, Penninga, Qian, Read, Reinetta M. Buitelaar, Richèle D. Wind, Sambrook, Sanger, Schmid, Starnes, Strokopytov, Strokopytov, Svensson, Takase, Takusagawa, Tronrud, Wind, Yanisch-Perron   +44 more
core   +2 more sources

Molecular Tweezers for Biomimetic Recognition of Carbohydrates

ChemBioChem, EarlyView.
Molecular tweezers have emerged as effective binders for biomolecules such as lipids, nucleic acids, and proteins, eliciting biological activities. Tweezer molecules, which exploit an open and adaptive binding cavity, can target relatively large guests, such as oligosaccharides and glycans, and often provide some advantages over corresponding ...
Francesco Milanesi, Naufia Mohamedzakaria Shibinasbarveen, Stefano Roelens, Oscar Francesconi   +3 more
wiley   +1 more source

Merkel cell polyomavirus large T antigen disrupts lysosome clustering by translocating human Vam6p from the cytoplasm to the nucleus [PDF]

, 2011
Merkel cell polyomavirus (MCV) has been recently described as the cause for most human Merkel cell carcinomas. MCV is similar to simian virus 40 (SV40) and encodes a nuclear large T (LT) oncoprotein that is usually mutated to eliminate viral replication ...
Ali, Binda, Campbell, Campbell, Caplan, Cotsiki, Côté-Martin, DeCaprio, Dilworth, Felici, Feng, Flinn, Hakki, Houben, Jennifer Hein, Lane, Laude, Li, Liang, Linzer, Mannová, Nakamura, Nakamura, Ole V. Gjoerup, Pallas, Patrick S. Moore, Pelkmans, Per H. Basse, Peralta, Pipas, Poupon, Price, Qian, Richardson, Schowalter, Shuda, Shuda, Simon C.W. Richardson, Srinivasan, Sukarieh, Tuna Toptan, Whitman, Wrana, Wurmser, Xi Liu, Yuan Chang   +45 more
core   +2 more sources

Deciphering the Electrochemical Activity and Selectivity of Earth‐Abundant Transition Metal‐Based Catalysts for the Alcohol Electrooxidation—Current Status

ChemCatChem, Volume 17, Issue 8, April 15, 2025.
This paper reviews the electrocatalytic activity and selectivity of non‐noble metal‐based catalysts in alternative anode reactions, particularly the glycerol oxidation reaction. It explores mechanistic factors influencing catalytic activity, including metal ions and oxygen species, emphasizing the importance of M─O motifs.
Michael Braun, Corina Andronescu
wiley   +1 more source

Develop machine learning based predictive models for engineering protein solubility [PDF]

arXiv, 2018
Protein activity is a significant characteristic for recombinant proteins which can be used as biocatalysts. High activity of proteins reduces the cost of biocatalysts. A model that can predict protein activity from amino acid sequence is highly desired, as it aids experimental improvement of proteins.
arxiv