Results 171 to 180 of about 940,374 (326)

Maltose-binding protein does not modulate the activity of maltoporin as a general porin in Escherichia coli [PDF]

, 1985
J M Brass, K. Bauer, U Ehmann, Winfried Boos   +3 more
openalex   +1 more source

Photoaffinity labeling coupled to MS to identify peptide biological partners: Secondary reactions, for better or for worse?

Mass Spectrometry Reviews, EarlyView.
Abstract Affinity photolabeling is a smart method to study noncovalent and transient interactions and provide a submolecular picture of the contacts between interacting partners. In this review, we will focus on the identification of peptide partners using photoaffinity labeling coupled to mass spectrometry in different contexts such as in vitro with a
Astrid Walrant, Emmanuelle Sachon
wiley   +1 more source

Protein Hypernetworks: a Logic Framework for Interaction Dependencies and Perturbation Effects in Protein Networks [PDF]

arXiv, 2011
Motivation: Protein interactions are fundamental building blocks of biochemical reaction systems underlying cellular functions. The complexity and functionality of such systems emerge not from the protein interactions themselves but from the dependencies between these interactions.
arxiv  

Electrokinetic Manipulations Combined With Direct and Ambient Ionization Mass Spectrometry

Mass Spectrometry Reviews, EarlyView.
ABSTRACT Mass spectrometry (MS) is a powerful analytical technique that typically involves sample preparation and online analytical separation before MS detection. Traditional methods often face bottlenecks in sample preparation and analytical separation, despite the rapid detection capabilities of MS.
Nicholas E. Manicke, Lahiru Wedasingha, Magnus Rydberg   +2 more
wiley   +1 more source

Preventive Effect of Upland Pigmented Potatoes Against LPS‐Induced Inflammation in THP‐1 Macrophages

Molecular Nutrition &Food Research, EarlyView.
The anti‐inflammatory activity of extracts from three commercial potato varieties cultivated upland was investigated on THP‐1 macrophages insulted with LPS. While the chlorogenic acid (CGA)‐rich extract from Kennebec reduced inflammation only at supraphysiological doses, carotenoid‐rich Desirèe and anthocyanin (ACN)‐rich Bleuet decreased TNF‐α, IL‐1β ...
Marta Toccaceli, Alessandra Marinelli, Federico Ballabio, Laura Bassolino, Roberto Lo Scalzo, Bruno Parisi, Daniela Pacifico, Federica Nicoletti, Carlo Camilloni, Giuseppe Mandolino, Katia Petroni   +10 more
wiley   +1 more source

Characterization of the E26H Mutant Schistosoma japonicum Glutathione S‐Transferase

Proteins: Structure, Function, and Bioinformatics, Volume 93, Issue 5, Page 1054-1066, May 2025.
ABSTRACT Glutathione‐S‐transferase, such as that of Schistosoma japonicum (sjGST) belongs to the most widely utilized fusion tags in the recombinant protein technology. The E26H mutation of sjGST has already been found to remarkably improve its ability for binding divalent ions, enabling its purification with immobilized metal affinity chromatography ...
János András Mótyán, Ágota Nagyné Veres, József Tőzsér   +2 more
wiley   +1 more source

Identifying Interaction Sites in "Recalcitrant" Proteins: Predicted Protein and Rna Binding Sites in Rev Proteins of Hiv-1 and Eiav Agree with Experimental Data [PDF]

arXiv, 2005
Protein-protein and protein nucleic acid interactions are vitally important for a wide range of biological processes, including regulation of gene expression, protein synthesis, and replication and assembly of many viruses. We have developed machine learning approaches for predicting which amino acids of a protein participate in its interactions with ...
arxiv  

The Pseudoenzyme β‐Amylase9 From Arabidopsis Activates α‐Amylase3: A Possible Mechanism to Promote Stress‐Induced Starch Degradation

Proteins: Structure, Function, and Bioinformatics, Volume 93, Issue 6, Page 1189-1201, June 2025.
ABSTRACT Starch accumulation in plants provides carbon for nighttime use, for regrowth after periods of dormancy, and for times of stress. Both ɑ‐ and β‐amylases (AMYs and BAMs, respectively) catalyze starch hydrolysis, but their functional roles are unclear.
Christopher E. Berndsen, Amanda R. Storm, Angelina M. Sardelli, Sheikh R. Hossain, Kristen R. Clermont, Luke M. McFather, Mafe A. Connor, Jonathan D. Monroe   +7 more
wiley   +1 more source

Modeling, virtual screening, and enzymatic docking of trehalose 6‐phosphate phosphatase and evaluation of the insecticidal effect of phthalimide, N‐(p‐tolylsulfonyl) on Aedes aegypti (Diptera: Culicidae)

Pest Management Science, EarlyView.
Schematic diagram of the experimental procedures used to perform the bioinformatic steps and insecticidal assays using trehalose 6‐phosphate phosphatase of Aedes aegypti and the molecule phthalimide, N‐(p‐tolylsulfonyl). Abstract BACKGROUND Aedes aegypti Linnaeus is a medically important vector because of its role in transmitting several arboviruses ...
Raquel Jemima Viana Lima, Fernando Berton Zanchi, Railton Marques de Souza Guimarães, Alexandre de Almeida e Silva   +3 more
wiley   +1 more source

Microarray-based evaluation of selected recombinant timothy grass allergens expressed in E. Coli and N. Benthamiana

BMC Biotechnology
Background Timothy grass (Phleum pratense) is a significant source of allergens, and recombinant allergens are increasingly used for diagnostic purposes.
Laimis Silimavicius, Lieve Tchebotarev, Mindaugas Zaveckas, Raimundas Razanskas, Laima Cepulyte, Karolina Bielske, Indre Kucinskaite-Kodze, Linas Griguola, Kotryna Linauskiene, Rasa Petraityte-Burneikiene   +9 more
doaj   +1 more source