Ligand-modulated Parallel Mechanical Unfolding Pathways of Maltose-binding Proteins [PDF]
Journal of Biological Chemistry, 2011 Protein folding and unfolding are complex phenomena, and it is accepted that multidomain proteins generally follow multiple pathways. Maltose-binding protein (MBP) is a large (a two-domain, 370-amino acid residue) bacterial periplasmic protein involved in maltose uptake.
Vasudha Aggarwal +5 more
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Structure of an engineered β-lactamase maltose binding protein fusion protein: insights into heterotropic allosteric regulation. [PDF]
PLoS ONE, 2012 Engineering novel allostery into existing proteins is a challenging endeavor to obtain novel sensors, therapeutic proteins, or modulate metabolic and cellular processes.
Wei Ke +8 more
doaj +3 more sources
Folding of maltose binding protein outside of and in GroEL. [PDF]
Proc Natl Acad Sci U S A, 2018 Significance The GroEL/ES chaperonin is known to prevent protein aggregation during folding by passive containment within the central cavity. The possible role of more active intervention is controversial. The HX MS method documents an organized hydrophobically stabilized folding preintermediate in the collapsed ensemble of maltose binding ...
Ye X, Mayne L, Kan ZY, Englander SW.
europepmc +4 more sources
In Silico Generation of Peptides by Replica Exchange Monte Carlo: Docking-Based Optimization of Maltose-Binding-Protein Ligands. [PDF]
PLoS ONE, 2015 Short peptides can be designed in silico and synthesized through automated techniques, making them advantageous and versatile protein binders. A number of docking-based algorithms allow for a computational screening of peptides as binders.
Anna Russo +8 more
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A Maltose-Binding Protein Fusion Construct Yields a Robust Crystallography Platform for MCL1. [PDF]
PLoS ONE, 2015 Crystallization of a maltose-binding protein MCL1 fusion has yielded a robust crystallography platform that generated the first apo MCL1 crystal structure, as well as five ligand-bound structures.
Matthew C Clifton +17 more
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Prokaryotic Soluble Overexpression and Purification of Human VEGF165 by Fusion to a Maltose Binding Protein Tag. [PDF]
PLoS ONE, 2016 Human vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis and plays a central role in the process of tumor growth and metastatic dissemination. Escherichia coli is one of the most common expression systems used for the production
Minh Tan Nguyen +13 more
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Correction: Prokaryotic soluble overexpression and purification of human VEGF165 by fusion to a maltose binding protein tag. [PDF]
PLoS ONE, 2017 [This corrects the article DOI: 10.1371/journal.pone.0156296.].
Minh Tan Nguyen +13 more
doaj +2 more sources
A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein. [PDF]
J Biomol NMR, 2021 Chae YK, Um Y, Kim H.
europepmc +3 more sources
Engineered Derivatives of Maltose-Binding Protein
, 2012 Maltose-binding protein (MBP), a member of the periplasmic binding protein family of Gram negative bacteria, is a versatile substrate for protein engineering. In common with other periplasmic proteins, it is extremely protease resistant, and it can fold properly in both the cytoplasmic and periplasmic compartments.
Dipak Paul
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Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins.
J Struct Biol, 2016 We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations that enhance MBP solubility and affinity purification ...
Bokhove M +9 more
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