Interaction of the maltose-binding protein with membrane vesicles of Escherichia coli [PDF]
Journal of Bacteriology, 1982 The interaction of the radioactively labeled purified maltose-binding protein of Escherichia coli with membrane vesicles was studied. The maltose-binding protein bound specifically to the vesicles, in the presence of maltose, on few sites. Under conditions in which a potential was imposed across the membrane, the specific binding was (i) increased, (ii)
G. Richarme
semanticscholar +4 more sources
A simple and sensitive detection of the binding ligands by using the receptor aggregation and NMR spectroscopy: a test case of the maltose binding protein. [PDF]
J Biomol NMR, 2021 Chae YK, Um Y, Kim H.
europepmc +3 more sources
Exploring the Unfolding Pathway of Maltose Binding Proteins: An Integrated Computational Approach [PDF]
Journal of Chemical Theory and Computation, 2014 Recent single-molecule force spectroscopy experiments on the Maltose Binding Proteins (MBPs) identified four stable structural units, termed unfoldons, that resist mechanical stress and determine the intermediates of the unfolding pathway. In this work, we analyze the topological origin and the dynamical role of the unfoldons using an integrated ...
Carlo Guardiani +4 more
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Precursor maltose-binding protein is active in binding substrate.
Journal of Biological Chemistry, 1979 Precursor maltose-binding protein synthesized in vitro was shown to be active in binding maltose by affinity chromatography.
Thomas Ferenci, Linda L. Randall
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Maltose-binding protein fusion allows for high level bacterial expression and purification of bioactive mammalian cytokine derivatives. [PDF]
PLoS ONE, 2014 Fusokines are chimeric proteins generated by the physical coupling of cytokines in a single polypeptide, resulting in proteins with highly pleiotropic activity and the potential to treat cancer and autoimmune ailments.
Andrea Pennati +2 more
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Affinity Purification of a Recombinant Protein Expressed as a Fusion with the Maltose-Binding Protein (MBP) Tag. [PDF]
Methods Enzymol, 2015 Duong-Ly KC, Gabelli SB.
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Expression of Tailored α-N-Acetylglucosaminidase in Escherichia coli for Synthesizing Mannose-6-Phosphate on N-Linked Oligosaccharides of Lysosomal Enzymes [PDF]
BioengineeringLysosomal enzymes are synthesized as N-glycosylated glycoproteins with mannose-6-phosphate (M6P) moieties, which are responsible for their binding to M6P receptors and transporting to the lysosome. In the M6P biosynthetic pathway, a Man8GlcNAc2 glycoform
Yunsong Cao, Wei Wang
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Full engagement of liganded maltose-binding protein stabilizes a semi-open ATP-binding cassette dimer in the maltose transporter. [PDF]
Mol Microbiol, 2015 Alvarez FJ +6 more
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Identification of a system required for the functional surface localization of sugar binding proteins with class III signal peptides in Sulfolobus solfataricus [PDF]
, 2007 The hyperthermophilic archaeon Sulfolobus solfataricus contains an unusual large number of sugar binding proteins that are synthesized as precursors with a class III signal peptide.
Albers S.V. +12 more
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Stability of ligand-induced protein conformation influences affinity in maltose-binding protein
bioRxiv, 2021 Our understanding of what determines ligand affinity of proteins is poor, even with high-resolution structures available. Both the non-covalent ligand-protein interactions and the relative free energies of available conformations contribute to the ...
Marco van den Noort +2 more
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