Ligand-modulated parallel mechanical unfolding pathways of maltose-binding proteins. [PDF]
J Biol Chem, 2011 Protein folding and unfolding are complex phenomena, and it is accepted that multidomain proteins generally follow multiple pathways. Maltose-binding protein (MBP) is a large (a two-domain, 370-amino acid residue) bacterial periplasmic protein involved in maltose uptake.
Aggarwal V +5 more
europepmc +6 more sources
Dynamical Signatures of Thermotoga maritima Maltose-Binding Proteins Affected by Ligand Binding [PDF]
ACS OmegaShantanu Shukla +6 more
doaj +3 more sources
Structure dictates the mechanism of ligand recognition in the histidine and maltose binding proteins [PDF]
Current Research in Structural Biology, 2020 Two mechanisms, induced fit (IF) and conformational selection (CS), have been proposed to explain ligand recognition coupled conformational changes. The histidine binding protein (HisJ) adopts the CS mechanism, in which a pre-equilibrium is established ...
Lakshmi P. Jayanthi +2 more
doaj +2 more sources
Structure of an engineered β-lactamase maltose binding protein fusion protein: insights into heterotropic allosteric regulation. [PDF]
PLoS ONE, 2012 Engineering novel allostery into existing proteins is a challenging endeavor to obtain novel sensors, therapeutic proteins, or modulate metabolic and cellular processes.
Wei Ke +8 more
doaj +3 more sources
Competitive interactions of ligands and macromolecular crowders with maltose binding protein. [PDF]
PLoS ONE, 2013 Cellular signaling involves a cascade of recognition events occurring in a complex environment with high concentrations of proteins, polysaccharides, and other macromolecules.
Andrew C Miklos +2 more
doaj +4 more sources
Accessing a hidden conformation of the maltose binding protein using accelerated molecular dynamics.
PLoS Computational Biology, 2011 Periplasmic binding proteins (PBPs) are a large family of molecular transporters that play a key role in nutrient uptake and chemotaxis in Gram-negative bacteria.
Denis Bucher +3 more
doaj +2 more sources
Pharmaceutics, 2022 Protein modification with non-canonical amino acids (ncAAs) represents a useful technology to afford homogenous samples of bioconjugates with site-specific modification.
Alexander C. Williard +10 more
doaj +1 more source
Discovery of an auto-regulation mechanism for the maltose ABC transporter MalFGK2. [PDF]
PLoS ONE, 2012 The maltose transporter MalFGK(2), together with the substrate-binding protein MalE, is one of the best-characterized ABC transporters. In the conventional model, MalE captures maltose in the periplasm and delivers the sugar to the transporter.
Huan Bao, Franck Duong
doaj +1 more source
In vitro recombination of non-homologous genes can result in gene fusions that confer a switching phenotype to cells. [PDF]
PLoS ONE, 2011 Regulation of protein activity is central to the complexity of life. The ability to regulate protein activity through exogenously added molecules has biotechnological/biomedical applications and offers tools for basic science.
Richard A Heins +3 more
doaj +1 more source
Heterologous expression of mycobacterial Esx complexes in Escherichia coli for structural studies is facilitated by the use of maltose binding protein fusions. [PDF]
PLoS ONE, 2013 The expression of heteroligomeric protein complexes for structural studies often requires a special coexpression strategy. The reason is that the solubility and proper folding of each subunit of the complex requires physical association with other ...
Mark A Arbing +14 more
doaj +1 more source