Results 171 to 180 of about 40,725 (187)

Induced fit of passenger proteins fused to Archaea maltose binding proteins

Biochemical and Biophysical Research Communications, 2006
Maltose binding proteins (MBPs) are used as carriers for improving the solubility of passenger proteins. Our results indicate that the higher solubility of the fusions correlates with their elevated heat stability. Fusions of the otherwise thermo-sensitive GFP with MBPs from Archaea, but not GST-GFP and Escherichia coli MBP-GFP, maintained their ...
He, Huang, Jing, Liu, Ario, de Marco
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Strategy for purifying maltose binding protein fusion proteins by affinity precipitation

Journal of Chromatography A, 2008
The maltose binding protein (MBP) affinity tag has been extensively used for protein purification. A commercial grade cationic starch could precipitate MBP or an MBP-tagged protein quantitatively by simultaneous addition of 10% (w/v) polyethylene glycol (PEG) and 50 mM calcium chloride.
Raghava, Smita, Aquil, Samina, Bhattacharyya, Sanchari, Varadarajan, Raghavan, Gupta, Munishwar N   +4 more
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Dynamical persistence of active sites identified in maltose-binding protein

Journal of Molecular Modeling, 2017
This study identifies dynamical properties of maltose-binding protein (MBP) useful in unveiling active site residues susceptible to ligand binding. The described methodology has been previously used in support of novel topological techniques of persistent homology and statistical inference in complex, multi-scale, high-dimensional data often ...
Dragan Nikolić, Violeta Kovačev-Nikolić   +1 more
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Tritium NMR spectroscopy of ligand binding to maltose-binding protein

Biochemistry, 1991
Tritium-labeled alpha- and beta-maltodextrins have been used to study their complexes with maltose-binding protein (MBP), a 40-kDa bacterial protein. Five substrates, from maltose to maltohexaose, were labeled at their reducing ends and their binding studied.
K, Gehring, P G, Williams, J G, Pelton, H, Morimoto, D E, Wemmer   +4 more
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DEVELOPMENT OF GENETICALLY ENCODED FLUORESCENT PROTEIN CONSTRUCTS OF HYPERTHERMOPHILIC MALTOSE-BINDING PROTEIN

Preparative Biochemistry and Biotechnology, 2013
Circularly permuted green fluorescent protein (cGFP) was inserted into the hyperthermophilic maltose binding protein at two different locations. cGFP was inserted between amino acid residues 206 and 207, or fused to the N-terminal of maltose binding protein from Thermotoga maritima.
Ozyurt C., Evran S., Telefoncu A.
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Export of the periplasmic maltose-binding protein ofEscherichia coli

Journal of Bioenergetics and Biomembranes, 1990
The export of the maltose-binding protein (MBP), the malE gene product, to the periplasm of Escherichia coli cells has been extensively investigated. The isolation of strains synthesizing MalE-LacZ hybrid proteins led to a novel genetic selection for mutants that accumulate export-defective precursor MBP (preMBP) in the cytoplasm.
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A convenient method for affinity purification of maltose binding protein fusions

Journal of Biotechnology, 1998
Maltose binding protein of Escherichia coli is frequently employed as a fusion partner for the biosynthesis of polypeptides and proteins, largely because of the advantage provided by affinity purification of such a fusion. A mixture of cellulose and starch is shown to be a simple and effective alternative to the use of other, more complicated media ...
U, Srinivasan, J A, Bell
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Maltodextrin-Modified Magnetic Microspheres for Selective Enrichment of Maltose Binding Proteins

ACS Applied Materials & Interfaces, 2014
In this work, maltodextrin-modified magnetic microspheres Fe3O4@SiO2-Maltodextrin (Fe3O4@SiO2-MD) with uniform size and fine morphology were synthesized through a facile and low-cost method. As the maltodextrins on the surface of microspheres were combined with maltose binding proteins (MBP), the magnetic microspheres could be applied to enriching ...
Jin, Zheng, Chongjun, Ma, Yangfei, Sun, Miaorong, Pan, Li, Li, Xiaojian, Hu, Wuli, Yang   +6 more
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A Fluorescence Resonance Energy Transfer Sensor Based on Maltose Binding Protein

Bioconjugate Chemistry, 2003
A fluorescence resonance energy-transfer (FRET) sensing system for maltose based on E. coli maltose binding protein (MBP) is demonstrated. The FRET donor portion of the sensing system consists of MBP modified with long wavelength-excitable cyanine dyes (Cy3 or Cy3.5).
Igor L, Medintz, Ellen R, Goldman, Michael E, Lassman, J Matthew, Mauro   +3 more
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Associative properties of the Escherichiacoli galactose binding protein and maltose binding protein

Biochemical and Biophysical Research Communications, 1982
The ligand-binding characteristics of periplasmic galactose-binding protein and maltose-binding protein of Escherichia coli are analyzed. The saturation function was decreased upon increasing protein concentration and the monomer-dimer equilibrium was shifted towards the monomeric protein form upon an increase of the ligand concentration.
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