Engineered Derivatives of Maltose-Binding Protein
, 2012 Maltose-binding protein (MBP), a member of the periplasmic binding protein family of Gram negative bacteria, is a versatile substrate for protein engineering.
Dipak Paul
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Discovery of an auto-regulation mechanism for the maltose ABC transporter MalFGK2. [PDF]
PLoS ONE, 2012 The maltose transporter MalFGK(2), together with the substrate-binding protein MalE, is one of the best-characterized ABC transporters. In the conventional model, MalE captures maltose in the periplasm and delivers the sugar to the transporter.
Huan Bao, Franck Duong
doaj +21 more sources
Maltoporins and maltose-binding proteins of Yersinia enterocolitica. [PDF]
Journal of General Microbiology, 1993 Two components of the Yersinia enterocolitica maltose transport system, maltoporin (OmpM) and an osmotically shockable periplasmic maltose-binding protein (MBP) were identified.
K. Brzostek, H. Heleszko, J. Hrebenda
semanticscholar +4 more sources
In vitro recombination of non-homologous genes can result in gene fusions that confer a switching phenotype to cells. [PDF]
PLoS ONE, 2011 Regulation of protein activity is central to the complexity of life. The ability to regulate protein activity through exogenously added molecules has biotechnological/biomedical applications and offers tools for basic science.
Richard A Heins +3 more
doaj +5 more sources
The ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediated. [PDF]
PLoS ONE, 2012 Escherichia coli maltose binding protein (MBP) is commonly used to promote the solubility of its fusion partners. To investigate the mechanism of solubility enhancement by MBP, we compared the properties of MBP fusion proteins refolded in vitro with ...
Sreejith Raran-Kurussi, David S Waugh
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Induced Fit or Conformational Selection? The Role of the Semi-closed State in the Maltose Binding Protein [PDF]
Biochemistry, 2011 A full characterization of the thermodynamic forces underlying ligand-associated conformational changes in proteins is essential for understanding and manipulating diverse biological processes, including transport, signaling, and enzymatic activity ...
Denis Bucher +2 more
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Defective secretion of maltose- and ribose-binding proteins caused by a truncated periplasmic protein in Escherichia coli [PDF]
Journal of Bacteriology, 1985 Regine Hengge, Winfried Boos
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Differential Regulation of Hyaluronan Synthesis by Three Isoforms of Hyaluronan Synthases in Mammalian Cells [PDF]
BiomoleculesHyaluronan (HA) is one of the crucial components of the extracellular matrix in vertebrates and is synthesized by three hyaluronan synthases (HASs), namely HAS1, HAS2, and HAS3.
Jie Wang +3 more
doaj +2 more sources
Identification of a system required for the functional surface localization of sugar binding proteins with class III signal peptides in Sulfolobus solfataricus [PDF]
, 2007 The hyperthermophilic archaeon Sulfolobus solfataricus contains an unusual large number of sugar binding proteins that are synthesized as precursors with a class III signal peptide.
Albers S.V. +12 more
core +28 more sources
The energetics of structural change in maltose-binding protein [PDF]
Proceedings of the National Academy of Sciences, 2003 High-resolution structures of proteins and their complexes are now appearing in the Research Collaboratory for Structural Bioinformatics (RCSB) database at a tremendous rate. When analyzing the structures of proteins, it is often tempting to try to extract energetic contributions to stability or binding interactions.
David E. Wemmer
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