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Protein Purification with C-Terminal Fusion of Maltose Binding Protein
Protein Expression and Purification, 1998For affinity-chromatography-based purification of proteins that are prone to abnormal termination of translation or that may not be modified at their N-termini, affinity tags are needed which can be fused to the C-terminus. In this publication we describe that maltose binding protein (MBP) fused to the C-terminus of the plant photoreceptor phytochrome ...
Lars Hennig, Eberhard Schäfer
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Journal of the American Chemical Society, 2001
TROSY-based HN(CO)CA 2D and 3D pulse schemes are presented for measurement of (13)C(alpha)-(13)C(beta) dipolar couplings in high molecular weight (15)N,(13)C,(2)H-labeled proteins.
J. Evenäs +3 more
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TROSY-based HN(CO)CA 2D and 3D pulse schemes are presented for measurement of (13)C(alpha)-(13)C(beta) dipolar couplings in high molecular weight (15)N,(13)C,(2)H-labeled proteins.
J. Evenäs +3 more
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[75] Maltose-binding protein from Escherichia coli
1982Publisher Summary This chapter describes the assay method, the purification procedure, and the properties of maltose-binding protein from Escherichia coli . The maltose-binding protein is a periplasmic protein of Escherichia coli that is involved in the transport of maltose and maltodextrins across the bacterial envelope and in the chemotaxis ...
Odile K. Kellermann, Thomas Ferenci
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Kinetics and Energetics of the Translocation of Maltose Binding Protein Folding Mutants
Journal of Molecular Biology, 2008Protein translocation in Escherichia coli is mediated by the translocase that, in its minimal form, comprises a protein-conducting pore (SecYEG) and a motor protein (SecA). The SecYEG complex forms a narrow channel in the membrane that allows passage of secretory proteins (preproteins) in an unfolded state only.
Tomikiewicz, Danuta +3 more
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Export of the periplasmic maltose-binding protein ofEscherichia coli
Journal of Bioenergetics and Biomembranes, 1990The export of the maltose-binding protein (MBP), the malE gene product, to the periplasm of Escherichia coli cells has been extensively investigated. The isolation of strains synthesizing MalE-LacZ hybrid proteins led to a novel genetic selection for mutants that accumulate export-defective precursor MBP (preMBP) in the cytoplasm.
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Dynamical persistence of active sites identified in maltose-binding protein
Journal of Molecular Modeling, 2017This study identifies dynamical properties of maltose-binding protein (MBP) useful in unveiling active site residues susceptible to ligand binding. The described methodology has been previously used in support of novel topological techniques of persistent homology and statistical inference in complex, multi-scale, high-dimensional data often ...
Dragan Nikolić +1 more
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Membrane Protein Topology Determination by Proteolysis of Maltose Binding Protein Fusions
Analytical Biochemistry, 1993A method is presented for determining the topology of Escherichia coli inner membrane proteins that is based on proteolysis of fusion proteins between maltose binding protein (MBP) and the membrane protein of interest. Fusion proteins are constructed wherein the MBP domain is fused upstream of the membrane protein domain.
J. Olson +3 more
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Induced fit of passenger proteins fused to Archaea maltose binding proteins
Biochemical and Biophysical Research Communications, 2006Maltose binding proteins (MBPs) are used as carriers for improving the solubility of passenger proteins. Our results indicate that the higher solubility of the fusions correlates with their elevated heat stability. Fusions of the otherwise thermo-sensitive GFP with MBPs from Archaea, but not GST-GFP and Escherichia coli MBP-GFP, maintained their ...
Ario de Marco +3 more
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A convenient method for affinity purification of maltose binding protein fusions
Journal of Biotechnology, 1998Maltose binding protein of Escherichia coli is frequently employed as a fusion partner for the biosynthesis of polypeptides and proteins, largely because of the advantage provided by affinity purification of such a fusion. A mixture of cellulose and starch is shown to be a simple and effective alternative to the use of other, more complicated media ...
Usha Srinivasan, Jeffrey A. Bell
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Folding and aggregation of export-defective mutants of the maltose-binding protein
Research in Microbiology, 2002We previously characterized a defective-folding variant of the periplasmic maltose-binding protein, MalE31. To examine the alternative folding pathways open to the MalE31 precursor, we have analyzed the cellular fates of this aggregation-prone protein carrying altered signal sequences.
Sabine Hunke +2 more
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