Results 21 to 30 of about 936,446 (303)
Pharmaceutics, 2022 Protein modification with non-canonical amino acids (ncAAs) represents a useful technology to afford homogenous samples of bioconjugates with site-specific modification.
Alexander C. Williard +10 more
doaj +1 more source
Thermodynamics of maltose binding protein unfolding [PDF]
Protein Science, 1997 AbstractThe maltose binding protein (MBP or MalE) of Escherichia coli is the periplasmic component of the transport system for malto‐oligosaccharides. It is used widely as a carrier protein for the production of recombinant fusion proteins. The melting of recombinant MBP was studied by differential scanning and titration calorimetry and fluorescence ...
Kenneth C. Ingham, Valery Novokhatny
openaire +3 more sources
Computational analysis of maltose binding protein translocation [PDF]
Philosophical Magazine, 2011 We propose a computational model for the study of maltose binding protein translocation across alpha-hemolysin nanopores. The phenomenological approach simplifies both the pore and the polypeptide chain; however it retains the basic structural protein-like properties of the maltose binding protein by promoting the correct formation of its native key ...
CHINAPPI, MAURO +2 more
openaire +5 more sources
Stability of ligand-induced protein conformation influences affinity in maltose-binding protein
bioRxiv, 2021 Our understanding of what determines ligand affinity of proteins is poor, even with high-resolution structures available. Both the non-covalent ligand-protein interactions and the relative free energies of available conformations contribute to the ...
Marco van den Noort +2 more
semanticscholar +1 more source
Backbone assignment of crystalline E. coli maltose binding protein [PDF]
Biomolecular NMR Assignments, 2021 The E.coli maltose binding protein (MBP) is a 42.5 kDa molecule widely employed in many biotechnology applications. Because of its molecular size, it has become the main model system for the development of solution NMR methods adapted to large biomolecular targets.
Tobias Schubeis +2 more
openaire +5 more sources
Easy mammalian expression and crystallography of maltose-binding protein-fused human proteins
Journal of Structural Biology, 2016 We present a strategy to obtain milligrams of highly post-translationally modified eukaryotic proteins, transiently expressed in mammalian cells as rigid or cleavable fusions with a mammalianized version of bacterial maltose-binding protein (mMBP). This variant was engineered to combine mutations that enhance MBP solubility and affinity purification ...
M. Bokhove +9 more
semanticscholar +5 more sources
Folding of maltose binding protein outside of and in GroEL [PDF]
Proceedings of the National Academy of Sciences, 2018 Significance The GroEL/ES chaperonin is known to prevent protein aggregation during folding by passive containment within the central cavity. The possible role of more active intervention is controversial. The HX MS method documents an organized hydrophobically stabilized folding preintermediate in the collapsed ensemble of maltose binding ...
Xiang Ye +3 more
openaire +3 more sources
Spectroscopic properties of an engineered maltose binding protein [PDF]
Protein Engineering Design and Selection, 1997 The maltose binding protein (MBP) has been site specifically labelled with a nitrobenzoxadiazole (NBD) group following mutation of a serine to a cysteine residue at position 337. The resulting protein shows a large ligand (maltose or beta-cyclodextrin) dependent increase in its steady-state fluorescence intensity.
GILARDI, Gianfranco +4 more
openaire +4 more sources
Role of Denaturation in Maltose Binding Protein Translocation Dynamics [PDF]
The Journal of Physical Chemistry B, 2012 We present a computational study on the driven transport of the Maltose Binding Protein (MBP) across nanochannels in the framework of coarse-grained modeling. The work is motivated by recent experiments on voltage-driven transport of MBP across nanopores exploring the influence of denaturation on translocation pathways. Our simplified approach allows a
Marco Bacci +3 more
openaire +7 more sources
Stubborn contaminants: influence of detergents on the purity of the multidrug ABC transporter BmrA. [PDF]
PLoS ONE, 2014 Despite the growing interest in membrane proteins, their crystallization remains a major challenge. In the course of a crystallographic study on the multidrug ATP-binding cassette transporter BmrA, mass spectral analyses on samples purified with six ...
Benjamin Wiseman +6 more
doaj +1 more source