Results 21 to 30 of about 40,725 (187)
Structural basis for negative regulation of the Escherichia coli maltose system
Nature Communications, 2023 Proteins from the signal transduction ATPases with numerous domains (STAND) family are known to play an important role in innate immunity. However, it remains less well understood how they function in transcriptional regulation. MalT is a bacterial STAND
Yuang Wu +4 more
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Role of Denaturation in Maltose Binding Protein Translocation Dynamics [PDF]
The Journal of Physical Chemistry B, 2012 We present a computational study on the driven transport of the Maltose Binding Protein (MBP) across nanochannels in the framework of coarse-grained modeling. The work is motivated by recent experiments on voltage-driven transport of MBP across nanopores exploring the influence of denaturation on translocation pathways. Our simplified approach allows a
Marco Bacci +3 more
openaire +6 more sources
Methods and Protocols, 2023 The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST).
Yuri Emoto +8 more
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Maltose-Binding Protein (MBP), a Secretion-Enhancing Tag for Mammalian Protein Expression Systems. [PDF]
PLoS ONE, 2016 Recombinant proteins are commonly expressed in eukaryotic expression systems to ensure the formation of disulfide bridges and proper glycosylation. Although many proteins can be expressed easily, some proteins, sub-domains, and mutant protein versions ...
Raphael Reuten +7 more
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eLife, 2021 With the recent explosion in high-resolution protein structures, one of the next frontiers in biology is elucidating the mechanisms by which conformational rearrangements in proteins are regulated to meet the needs of cells under changing conditions ...
William N Zagotta +9 more
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Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS
BioTechniques, 2010 We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies.
Hu Tao +5 more
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Expression and Purification of the Arabidopsis E4 SUMO Ligases PIAL1 and PIAL2
Bio-Protocol, 2015 The proteins PIAL1 (At1g08910) and PIAL2 (At5g41580) are members of the recently discovered group of plant E4 SUMO ligases. This protocol allows quick and simple expression of the recombinant proteins in Escherichia coli (E.
Konstantin Tomanov, Andreas Bachmair
doaj +1 more source
BioTechniques, 2022 Antibody (Ab) validation is the procedure in which an Ab is thoroughly assayed for sensitivity and specificity in a given application. Validation of Abs against post-translationally modified (PTM) targets is particularly challenging because it requires ...
Kezzia S Jones +10 more
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UV difference spectroscopy of ligand binding to maltose‐binding protein [PDF]
FEBS Letters, 1992 We have used UV absorbance spectroscopy to study the binding of linear and circular maltodextrins to maltose‐binding protein (MBP). Titrations with maltose yield three ??? points in the difference spectrum of MBP, consistent with two protein conformations: ligand‐free and ligand‐bound.
Gehring, Kalle +2 more
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Synthesis of fluorinated maltose derivatives for monitoring protein interaction by 19F NMR
Beilstein Journal of Organic Chemistry, 2012 A novel reporter system, which is applicable to the 19F NMR investigation of protein interactions, is presented. This approach uses 2-F-labeled maltose as a spy ligand to indirectly probe protein–ligand or protein–protein interactions of proteins fused ...
Michaela Braitsch +6 more
doaj +1 more source