Structural basis for negative regulation of the Escherichia coli maltose system
Nature Communications, 2023 Proteins from the signal transduction ATPases with numerous domains (STAND) family are known to play an important role in innate immunity. However, it remains less well understood how they function in transcriptional regulation. MalT is a bacterial STAND
Yuang Wu +4 more
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Methods and Protocols, 2023 The detection and quantification of protein–protein interactions (PPIs) is a crucial technique that often involves the use of recombinant proteins with fusion protein tags, such as maltose-binding protein (MBP) and glutathione-S-transferase (GST).
Yuri Emoto +8 more
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eLife, 2021 With the recent explosion in high-resolution protein structures, one of the next frontiers in biology is elucidating the mechanisms by which conformational rearrangements in proteins are regulated to meet the needs of cells under changing conditions ...
William N Zagotta +9 more
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Crystallization, data collection and data processing of maltose-binding protein (MalE) from the phytopathogen Xanthomonas axonopodis pv. citri [PDF]
, 2009 Maltose-binding protein is the periplasmic component of the ABC transporter responsible for the uptake of maltose/maltodextrins. The Xanthomonas axonopodis pv.
Balan, A. +4 more
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Dependence of protein-induced lipid bilayer deformations on protein shape [PDF]
, 2022 Membrane proteins typically deform the surrounding lipid bilayer membrane, which can play an important role in the function, regulation, and organization of membrane proteins. Membrane elasticity theory provides a beautiful description of protein-induced lipid bilayer deformations, in which all physical parameters can be directly determined from ...
arxiv +1 more source
Two closely related ABC transporters in streptococcus mutans are involved in disaccharide and/or oligosaccharide uptake. [PDF]
, 2007 Streptococcus mutans has a large number of transporters apparently involved in the uptake of carbohydrates. At least two of these, the multiple sugar metabolism transporter, MsmEFGK, and the previously uncharacterized MalXFGK, are members of the ATP ...
Homer, Karen A. +2 more
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Substrate induction and glucose repression of maltose utilization by Streptomyces coelicolor A3(2) is controlled by malR, a member of the lacI-galR family of regulatory genes [PDF]
, 1997 malR of Strepomyces coelicolor A3(2) encodes a homologue of the Lacl/Galr family of repressor proteins, and is divergently transcribed from the malEFG gene cluster, which encodes components of an ATP-dependent transport system that is required for ...
Bibb, M.J. +4 more
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Purifying natively folded proteins from inclusion bodies using sarkosyl, Triton X-100, and CHAPS
BioTechniques, 2010 We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies.
Hu Tao +5 more
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A useful epitope tag derived from maltose binding protein [PDF]
Protein Science, 2021 AbstractMaltose binding protein (MBP) is used in recombinant protein expression as an affinity and solubility tag. The monoclonal antibody B48 binds MBP tightly and has no cross‐reactivity to other proteins in an Escherichia coli lysate. This high level of specificity suggested that MBP contains an epitope that could prove useful as a purification and ...
Na Ke +7 more
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Structural basis for the mechanism of ABC transporters. [PDF]
, 2015 The ATP-binding cassette (ABC) transporters are primary transporters that couple the energy stored in adenosine triphosphate (ATP) to the movement of molecules across the membrane.
Beis, K
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