Results 31 to 40 of about 40,725 (187)
Frontiers in Physics, 2016 ATP-binding cassette (ABC) transporters are integral membrane proteins which mediate the exchange of diverse substrates across membranes powered by ATP molecules.
Holger eFlechsig
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Maltose binding protein-fusion enhances the bioactivity of truncated forms of pig myostatin propeptide produced in E. coli. [PDF]
PLoS ONE, 2017 Myostatin (MSTN) is a potent negative regulator of skeletal muscle growth. MSTN propeptide (MSTNpro) inhibits MSTN binding to its receptor through complex formation with MSTN, implying that MSTNpro can be a useful agent to improve skeletal muscle growth ...
Sang Beum Lee +2 more
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Protein quality control in the bacterial periplasm
Microbial Cell Factories, 2004 The proper functioning of extracytoplasmic proteins requires their export to, and productive folding in, the correct cellular compartment. All proteins in Escherichia coli are initially synthesized in the cytoplasm, then follow a pathway that depends ...
Betton Jean-Michel, Miot Marika
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Microbial Cell Factories, 2021 Background Proteins with novel functions or advanced activities developed by various protein engineering techniques must have sufficient solubility to retain their bioactivity.
Hyunjun Ko +7 more
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Folding of maltose binding protein outside of and in GroEL [PDF]
Proceedings of the National Academy of Sciences, 2018 Significance The GroEL/ES chaperonin is known to prevent protein aggregation during folding by passive containment within the central cavity. The possible role of more active intervention is controversial. The HX MS method documents an organized hydrophobically stabilized folding preintermediate in the collapsed ensemble of maltose binding ...
Xiang Ye +3 more
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The malQ gene is essential for starch metabolism in Streptococcus mutans
Journal of Oral Microbiology, 2013 Background: The malQ and glgP genes, respectively, annotated as putative 4-α-glucanotransferase and putative glycogen phosphorylase are located with a 29 nucleotide overlap on the Streptococcus mutans genome.
Yutaka Sato +2 more
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Visualizing conformational dynamics of proteins in solution and at the cell membrane
eLife, 2018 Conformational dynamics underlie enzyme function, yet are generally inaccessible via traditional structural approaches. FRET has the potential to measure conformational dynamics in vitro and in intact cells, but technical barriers have thus far limited ...
Sharona E Gordon +2 more
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BMC Biotechnology, 2020 Background Few bead-based multiplex assays have been described that detect antibodies against the protozoan parasite Toxoplasma gondii in large-scale seroepidemiological surveys. Moreover, each multiplex assay has specific variations or limitations, such
Sandra Klein, Daniel Stern, Frank Seeber
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BioTechniques, 2012 We observed the presence of contaminating NADH oxidation activity in maltose binding protein (MBP) fusion proteins expressed in Escherichia coli and purified using conventional amylose resin-based affinity chromatography.
Fengguang Guo, Guan Zhu
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Evidence for a "wattle and daub" model of the cyst wall of entamoeba. [PDF]
PLoS Pathogens, 2009 The cyst wall of Entamoeba invadens (Ei), a model for the human pathogen Entamoeba histolytica, is composed of fibrils of chitin and three chitin-binding lectins called Jacob, Jessie3, and chitinase.
Anirban Chatterjee +6 more
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