Results 41 to 50 of about 40,725 (187)
Maltose-binding protein is a potential carrier for oral immunizations [PDF]
Veterinary Immunology and Immunopathology, 2013 Maltose binding protein (MBP) is often fused to a relevant protein to improve its yield and facilitate its purification, but MBP can also enhance the immunogenicity of the fused proteins. Recent data suggest that MBP may potentiate antigen-presenting functions in immunized animals by providing intrinsic maturation stimuli to dendritic cells through ...
P, Bellot +5 more
openaire +2 more sources
Scientific Reports, 2021 Cysteine-rich secretory proteins (CRISPs) are a subgroup of the CRISP, antigen 5 and PR-1 (CAP) superfamily that is characterized by the presence of a conserved CAP domain.
Jie Sheng +4 more
doaj +1 more source
The ability to enhance the solubility of its fusion partners is an intrinsic property of maltose-binding protein but their folding is either spontaneous or chaperone-mediated. [PDF]
PLoS ONE, 2012 Escherichia coli maltose binding protein (MBP) is commonly used to promote the solubility of its fusion partners. To investigate the mechanism of solubility enhancement by MBP, we compared the properties of MBP fusion proteins refolded in vitro with ...
Sreejith Raran-Kurussi, David S Waugh
doaj +1 more source
Natural Products and Bioprospecting, 2018 Noncovalent interactions between ligands and targeting proteins are essential for understanding molecular mechanisms of proteins. In this work, we investigated the interaction of Cytochrome c (Cyt c) with maltoligosaccharides, namely maltose (Mal II ...
Quan Chi, Ying-Zhi Liu, Xian Wang
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Solution NMR studies of periplasmic binding proteins and their interaction partners
Biomolecular Concepts, 2011 Periplasmic binding proteins (PBPs) are a crucial part of ATP-binding cassette import systems in Gram-negative bacteria. Central to their function is the ability to undergo a large-scale conformational rearrangement from open-unliganded to closed ...
Pistolesi Sara +2 more
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Virology Journal, 2005 Background Replication of the vaccinia virus genome occurs in cytoplasmic factory areas and is dependent on the virus-encoded DNA polymerase and at least four additional viral proteins.
Moss Bernard, De Silva Frank S
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Frontiers in Bioengineering and Biotechnology, 2020 Steroid hormones that serve as vital compounds are necessary for the development and metabolism of a variety of organisms. The neverland (NVD) family genes encode the conserved Rieske-type oxygenases, which are accountable for the dehydrogenation during ...
Shuhong Mao +14 more
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Expression and purification of soluble and active human enterokinase light chain in Escherichia coli
Biotechnology Reports, 2021 Human enterokinase light chain (hEKL) specifically cleaves the sequence (Asp)4-Lys↓X (D4K), making this a frequently used enzyme for site-specific cleavage of recombinant fusion proteins.
Young Su Kim +4 more
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Bio-Protocol, 2020 Adeno-associated virus (AAV) is a promising gene therapy vector and the biophysical characterization of its interactions with host proteins is a critical foundation for engineering tissue targeting and immune escape.
Nancy Meyer +6 more
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Frontiers in Molecular Biosciences, 2021 LuxR, a bacterial quorum sensing-related transcription factor that responds to the signaling molecule 3-oxo-hexanoyl-homoserine lactone (3OC6-HSL). In this study, we employed molecular dynamics simulation and the Molecular Mechanics Generalized Born ...
Jinyu Li +14 more
doaj +1 more source